Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

CRISPR/Cas9 Genome Editing

CRISPR/Cas9 Genome Editing

The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...

CRISPR

CRISPR

Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...

CRISPR

CRISPR

CRISPR and crRNAs

CRISPR and crRNAs

Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Bacterial lipid synthesizing enzymes PlsY and PlsC utilize both stereo-forms of glycerol-phosphate.

EMBO reports·2026

Same author

Engineering Escherichia coli for the production of saturated archaeal lipids.

Metabolic engineering·2025

Same author

On the role of dcm and vsr in Escherichia coli adaptive mutation.

Molecular genetics and genomics : MGG·2025

Same author

Photoautotrophic Growth and the Transcriptome of the Halotolerant Model Microalga Dunaliella salina MCC43 Under Salt Stress.

Physiologia plantarum·2025

Same author

RNA extraction-free CRISPR-based SARS-CoV-2 detection in viral transport medium and dry swab-a comparative analysis: Short title: Extraction-free CRISPR-based Covid detection.

Diagnostic microbiology and infectious disease·2025

Same author

Cas11 augments Cascade functions in type I-E CRISPR system but is redundant for gene silencing and plasmid interference.

The Biochemical journal·2025

Same journal

Correction to 'New origin firing is inhibited by APC/CCdh1 activation in S-phase after severe replication stress'.

Nucleic acids research·2026

Same journal

VeloRM: disentangling pre- and post-splicing RNA modification dynamics at single-cell resolution.

Nucleic acids research·2026

Same journal

Accessibility of telomeric overhangs to stabilizing small-molecule ligands.

Nucleic acids research·2026

Same journal

Multivalent interactions mediate SNAIL transcription factor stimulation of the nucleosome deacetylase activity of the CoREST complex.

Nucleic acids research·2026

Same journal

Genome-wide mapping of DNA G-quadruplexes in Trypanosoma brucei chromatin reveals enrichment in coding regions and transcription start sites.

Nucleic acids research·2026

Same journal

Correction to 'The Gene Ontology knowledgebase in 2026'.

Nucleic acids research·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Apr 20, 2026

A New Toolkit for Evaluating Gene Functions using Conditional Cas9 Stabilization

A New Toolkit for Evaluating Gene Functions using Conditional Cas9 Stabilization

Published on: September 2, 2021

Efficient programmable gene silencing by Cascade.

Devashish Rath¹, Lina Amlinger¹, Mirthe Hoekzema¹

¹Department of Cell and Molecular Biology, Uppsala University, SE-751 24 Uppsala, Sweden.

Nucleic Acids Research

|December 2, 2014

Summary

This summary is machine-generated.

The CRISPR-associated Cascade complex offers efficient, programmable gene silencing by targeting DNA sequences. This adaptable technology enables precise control over gene expression for research and biotechnology applications.

More Related Videos

In Vitro Selection of Engineered Transcriptional Repressors for Targeted Epigenetic Silencing

In Vitro Selection of Engineered Transcriptional Repressors for Targeted Epigenetic Silencing

Published on: May 5, 2023

Selection-dependent and Independent Generation of CRISPR/Cas9-mediated Gene Knockouts in Mammalian Cells

Selection-dependent and Independent Generation of CRISPR/Cas9-mediated Gene Knockouts in Mammalian Cells

Published on: June 16, 2017

Related Experiment Videos

Last Updated: Apr 20, 2026

A New Toolkit for Evaluating Gene Functions using Conditional Cas9 Stabilization

A New Toolkit for Evaluating Gene Functions using Conditional Cas9 Stabilization

Published on: September 2, 2021

In Vitro Selection of Engineered Transcriptional Repressors for Targeted Epigenetic Silencing

In Vitro Selection of Engineered Transcriptional Repressors for Targeted Epigenetic Silencing

Published on: May 5, 2023

Selection-dependent and Independent Generation of CRISPR/Cas9-mediated Gene Knockouts in Mammalian Cells

Selection-dependent and Independent Generation of CRISPR/Cas9-mediated Gene Knockouts in Mammalian Cells

Published on: June 16, 2017

Area of Science:

Molecular Biology
Genetics
Biotechnology

Background:

Controlled gene expression is crucial for biological research and biotechnology.
Conventional methods often target individual genes or their regulatory elements.
CRISPR-based technologies offer new avenues for genetic manipulation.

Purpose of the Study:

To demonstrate the utility of the CRISPR-associated Cascade complex for programmable gene silencing.
To explore the efficiency and longevity of Cascade-mediated gene silencing.
To assess the adaptability of the system across different targets and organisms.

Main Methods:

Utilizing the CRISPR-associated DNA-binding Cascade complex for gene silencing.
Targeting Cascade to promoter sequences to inhibit transcription.
Targeting Cascade to open reading frame (ORF) sequences for gene silencing.
Employing a crRNA component for specific DNA binding.

Main Results:

Cascade effectively inhibits gene transcription when targeted to promoter regions, leading to reduced gene expression.
Cascade can also silence gene expression by targeting ORF sequences, though with lower efficiency.
The system demonstrates efficient, long-lasting, and programmable gene silencing.
The system successfully targets both plasmid and chromosomal DNA, can silence multiple genes simultaneously, and functions across various bacterial species and strains.

Conclusions:

The CRISPR-associated Cascade complex is a powerful tool for efficient and programmable gene silencing.
This technology expands the CRISPR toolbox and holds potential for adaptation to diverse organisms and cell systems.
The findings offer new possibilities for genetic research and biotechnological applications.