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Bead Aggregation Assays for the Characterization of Putative Cell Adhesion Molecules
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Protopine inhibits heterotypic cell adhesion in MDA-MB-231 cells through down-regulation of multi-adhesive factors

Kai He1, Jian-Li Gao2

  • 1The First Affiliated Hospital of Zhejiang University, Hangzhou, Zhejiang 310009, P.R. China ; Zhejiang Chinese Medical University, Hangzhou, Zhejiang 310053, P.R. China.

African Journal of Traditional, Complementary, and Alternative Medicines : AJTCAM
|December 2, 2014
PubMed
Abstract

Insights

Protopine, an alkaloid from Corydalis yanhusuo, inhibits breast cancer cell adhesion and invasion by reducing key adhesion molecule expression, suggesting a role in anti-metastasis therapy.

Area of Science:

  • Phytochemistry
  • Cancer Biology
  • Molecular Pharmacology

Background:

  • Previous studies indicated anticancer and anti-angiogenesis effects of Corydalis yanhusuo.
  • This study investigates the anticancer proliferation and adhesion effects of five alkaloids isolated from the herb.

Purpose of the Study:

  • To evaluate the anti-cancer effects of Corydalis yanhusuo alkaloids on breast cancer cells.
  • To identify specific alkaloids responsible for anti-proliferative and anti-adhesive properties.

Main Methods:

  • Utilized MTT assays, cell migration, invasion, and adhesion assays on MDA-MB-231 cells.
  • Investigated the mechanism of action by assessing the expression of EGFR, ICAM-1, and integrins via Western blotting.

Main Results:

  • Protopine demonstrated significant anti-adhesive and anti-invasion effects on MDA-MB-231 cells.
  • Treatment with protopine markedly reduced the expression of EGFR, ICAM-1, αv-integrin, β1-integrin, and β5-integrin.

Conclusions:

  • Protopine inhibits heterotypic cell adhesion between breast cancer cells and endothelial cells.
  • The mechanism involves modulating the expression of critical adhesion factors, contributing to anti-metastasis.
Keywords:
AdhesionBreast cancerCorydalis yanhusuoIntegrinsProtopine

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