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Updated: Apr 20, 2026

Quantification of Bacterial Histidine Kinase Autophosphorylation Using a Nitrocellulose Binding Assay
Published on: January 11, 2017
Bacterial histidine kinases as novel antibacterial drug targets
Agnieszka E Bem1, Nadya Velikova, M Teresa Pellicer
1Host-Microbe Interactomics, Wageningen University , De Elst 1, 6708 WD Wageningen, The Netherlands.
Abstract:
Bacterial histidine kinases (HKs) are promising targets for novel antibacterials. Bacterial HKs are part of bacterial two-component systems (TCSs), the main signal transduction pathways in bacteria, regulating various processes including virulence, secretion systems and antibiotic resistance. In this review, we discuss the biological importance of TCSs and bacterial HKs for the discovery of novel antibacterials, as well as published TCS and HK inhibitors that can be used as a starting point for structure-based approaches to develop novel antibacterials.
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Major types that are helpful drug targets include:

