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Related Experiment Videos

Diminished antigen processing by endosomal acidification mutant antigen-presenting cells.

K L McCoy1, J Miller, M Jenkins

  • 1Laboratory of Cellular and Molecular Immunology, National Institute of Allergy and Infectious Diseases, Bethesda, MD 20892.

Journal of Immunology (Baltimore, Md. : 1950)
|July 1, 1989
PubMed
Summary
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Acidification of early endosomes is crucial for antigen (Ag) processing by antigen-presenting cells (APCs). Impaired endosomal acidification in mutant cells significantly reduced their ability to process native protein antigens.

Area of Science:

  • Immunology
  • Cell Biology
  • Molecular Biology

Background:

  • Antigen processing is essential for adaptive immune responses, involving the breakdown of antigens into peptides for presentation by MHC class II molecules.
  • Endosomes are key organelles in antigen processing, and their acidification is thought to play a critical role in this pathway.

Purpose of the Study:

  • To investigate the specific role of early endosomal acidification in the processing of protein antigens by antigen-presenting cells (APCs).
  • To determine if impaired endosomal acidification affects the ability of APCs to stimulate T cells.

Main Methods:

  • Utilized temperature-sensitive mutant Chinese hamster ovary (CHO) cells with defects in endosomal acidification.
  • Transfected mutant CHO cells with MHC class II genes to create antigen-presenting cells.

Related Experiment Videos

  • Assessed antigen processing and T cell stimulation at permissive and non-permissive temperatures.

    • Main Results:

    • Mutant APCs incubated at the non-permissive temperature showed impaired processing of native protein antigens due to defective early endosomal acidification.
    • T cell stimulation was unaffected when mutant APCs presented pre-processed antigenic peptide fragments.
    • Uptake and overall degradation of antigens were not compromised in the mutant cells.

    Conclusions:

    • Acidification of early endosomes is a critical and necessary step for the physiological processing of native protein antigens.
    • This finding highlights the importance of endosomal pH regulation in initiating effective adaptive immune responses.