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Manipulation and Analysis of Cell Cycle-Dependent Processes in Budding Yeast
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Sequential feedback induction stabilizes the phosphate starvation response in budding yeast.

Noam Vardi1, Sagi Levy1, Yonat Gurvich1

  • 1Department of Molecular Genetics, Weizmann Institute of Science, Rehovot 76100, Israel.

Cell Reports
|December 2, 2014
PubMed
Summary
This summary is machine-generated.

Cells adapt to nutrient scarcity through gene expression changes. Yeast cells show two distinct gene induction waves during phosphate starvation, revealing how gene dynamics ensure stable adaptation.

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Area of Science:

  • Cellular Biology
  • Molecular Biology
  • Systems Biology

Background:

  • Nutrient depletion activates cellular regulatory programs for survival.
  • These programs enhance nutrient uptake, storage, and recycling.
  • Intracellular nutrient increase can create feedback instability.

Purpose of the Study:

  • To investigate how cells maintain stability during nutrient starvation.
  • To analyze the transcriptional response of budding yeast to phosphate depletion.
  • To understand the role of gene expression dynamics in cellular adaptation.

Main Methods:

  • Budding yeast (Saccharomyces cerevisiae) were transferred to phosphate-deficient medium.
  • Transcriptional response was monitored over time.
  • Gene expression patterns were analyzed in relation to nutrient availability and cell growth.

Main Results:

  • Phosphate starvation induced genes in two distinct temporal waves.
  • The first wave of gene induction was stable, even after phosphate replenishment, suggesting positive feedback.
  • The second wave, appearing after 2 hours, coincided with reduced growth and abolished the stable commitment, indicating dynamic regulation.

Conclusions:

  • Cellular adaptation to nutrient stress relies on the temporal dynamics of gene expression.
  • Sequential gene induction patterns are crucial for maintaining stability and optimizing adaptation.
  • Gene expression dynamics play a key role in preventing feedback instability during starvation response.