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Updates in inducible transgene expression using viral vectors: from transient to stable expression
Cara L Mortimer1, Benjamin Dugdale1, James L Dale1
1Centre for Tropical Crops and Biocommodities, Queensland University of Technology, Brisbane, Queensland 4000, Australia.
Current Opinion in Biotechnology
|December 2, 2014
Summary
Viral vectors enable cost-effective biologic production in plants. Advanced stable, inducible systems offer amplified gene expression for manufacturing diverse products in crops.
Area of Science:
- Plant biotechnology
- Molecular biology
- Bioprocessing
Background:
- Viral vectors have emerged as powerful tools for transiently amplifying transgene expression in plants.
- This has spurred interest in developing stable plant expression systems for biologics manufacturing.
- Adapting viral machinery for stable expression requires novel strategies for controlling gene activity.
Purpose of the Study:
- To develop advanced stable, inducible hyper-expression vectors for plant-based biologics production.
- To enable activated and amplified heterologous transgene expression in stably transformed plants.
- To explore the potential of broad acre crops for manufacturing a wide spectrum of products.
Main Methods:
- Deconstructing viral machinery for stable plant integration.
- Linking viral expression and replication to chemically controlled promoters.
- Engineering stable, inducible hyper-expression vectors.
Main Results:
- Development of advanced stable, inducible hyper-expression vectors.
- Demonstration of activated and amplified heterologous transgene expression.
- Potential for deployment in broad acre crops for biologics manufacturing.
Conclusions:
- Stable, inducible viral vector systems offer a promising pathway for economical plant-based biologics production.
- These advanced systems can significantly enhance the manufacturing capabilities of plants.
- Exploiting these technologies in crops could revolutionize the production of diverse bioproducts.
