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Published on: May 27, 2011
Enhanced expression of the Epstein-Barr virus latent membrane protein by a recombinant vaccinia virus
J P Stewart1, I N Hampson, H W Heinrich
1Paterson Institute for Cancer Research, Manchester, U.K.
Abstract:
The complete coding sequence of the Epstein-Barr virus strain B95-8 latent membrane protein (LMP) was cloned using a Raji cell cDNA library and genomic B95-8 DNA. The clone was characterized by sequencing and then used to make a recombinant vaccinia virus. This virus (VLMP) was shown to express a relatively high level of LMP in an authentic fashion. Antisera raised in rabbits against VLMP were shown to react with B95-8 LMP as well as cross-reacting with a 50K cellular protein.
