Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Endothelium as a transducing surface.

U S Ryan1

  • 1University of Miami, School of Medicine, Florida 33101.

Journal of Molecular and Cellular Cardiology
|February 1, 1989
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Receptor-dependent regulation of [Ca2+]i and phospholipase C in vascular endothelial cells.

Journal of receptor and signal transduction research·2001
Same author

Vaccine-induced antibodies inhibit CETP activity in vivo and reduce aortic lesions in a rabbit model of atherosclerosis.

Arteriosclerosis, thrombosis, and vascular biology·2000
Same author

Blockade of complement inhibits obliterative bronchiolitis in rat tracheal allografts.

American journal of respiratory and critical care medicine·2000
Same author

Spontaneous combined hyperlipidemia, coronary heart disease and decreased survival in Dahl salt-sensitive hypertensive rats transgenic for human cholesteryl ester transfer protein.

Nature medicine·1999
Same author

Endothelial targeting and enhanced antiinflammatory effects of complement inhibitors possessing sialyl Lewisx moieties.

Journal of immunology (Baltimore, Md. : 1950)·1999
Same author

Studies on the mechanisms involved in antigen-evoked pleural inflammation in rats: contribution of IgE and complement.

Journal of leukocyte biology·1997
Same journal

Large extracellular vesicles derived from red blood cells in coronary artery disease patients with anemia promote endothelial dysfunction.

Journal of molecular and cellular cardiology·2026
Same journal

SnRNA-seq identifies FN1-SDC4 axis triggering epicardial activation in right ventricular remodeling in pulmonary hypertension.

Journal of molecular and cellular cardiology·2026
Same journal

Therapeutic impact of normal dietary patterns on diabetic cardiomyopathy: Transcriptomic and proteomic insights.

Journal of molecular and cellular cardiology·2026
Same journal

Structural, biophysical and cellular assessment of filamin C M82K: A test case for VUS interpretation in cardiomyopathy.

Journal of molecular and cellular cardiology·2026
Same journal

Precision modification of heart failure signaling by CRISPR-Cas9 base editing.

Journal of molecular and cellular cardiology·2026
Same journal

Retraction notice to 'Sam68 impedes the recovery of arterial injury by augmenting inflammatory response' [Journal of Molecular and Cellular Cardiology 137 (2019) 82-92].

Journal of molecular and cellular cardiology·2026
See all related articles

Endothelial cells release vasodilators like endothelium-dependent relaxing factor (EDRF) and prostacyclin (PGI2), impacting vascular smooth muscle and platelets. Signal transduction studies reveal intracellular calcium elevation and protein kinase C activation in response to various stimuli.

Area of Science:

  • Cell biology
  • Vascular biology
  • Signal transduction

Background:

  • Endothelial cells produce vasodilators such as endothelium-dependent relaxing factor (EDRF) and prostacyclin (PGI2).
  • These mediators influence vascular smooth muscle relaxation and platelet aggregation.
  • Understanding the underlying signal transduction mechanisms is crucial.

Purpose of the Study:

  • To investigate the signal transduction pathways involved in endothelial cell responses.
  • To elucidate the role of intracellular calcium and protein kinase C in these processes.

Main Methods:

  • Intracellular calcium measurements using FURA-2 and INDO-1.
  • 45Ca flux techniques to quantify calcium content.
  • Patch clamp studies to identify ionic channels.

Related Experiment Videos

  • Investigation of phosphoinositide turnover and protein kinase C activation.

    • Main Results:

    • Vasodilator agonists transiently elevate intracellular calcium.
    • Bradykinin and thrombin increase endothelial cell calcium content.
    • Receptor activation stimulates phosphoinositide turnover and activates protein kinase C.
    • Receptor-operated ionic channels are present in the endothelial cell membrane.
    • Phagocytosis of bacteria by endothelial cells leads to superoxide anion generation, calcium elevation, and protein kinase C activation.
    • Phagocytosis also enhances endothelial cell migration, division, and further phagocytic activity.

    Conclusions:

    • Endothelial cell activation involves intracellular calcium elevation from both intracellular stores and extracellular influx.
    • Protein kinase C activation plays a role in feedback regulation and response to phagocytosis.
    • Endothelial cells exhibit complex and multipotent responses to diverse stimuli, including particulate matter.