[Antimutagenesis of multiphytoadaptogene in yeast saccharomyces]

Area of Science:

• Pharmacology
• Genetics
• Molecular Biology

Background:

• Multiphytoadaptogene (MPA) is a complex preparation derived from plant extracts, containing adaptogenic compounds.
• Genotoxicity studies are crucial for evaluating the safety and potential therapeutic effects of natural compounds.

Purpose of the Study:

• To investigate the antimutagenic activity of MPA against genotoxic agents.
• To elucidate the mechanisms underlying MPA's potential protective effects against DNA damage.

Main Methods:

• Genotoxicity assays were performed to assess the effect of MPA on induced mutagenesis in yeast models (ADE4-ADE8 loci).
• The study evaluated MPA's impact on mutations induced by UV radiation and nitrous acid.
• MPA's influence on different types of mutagenesis, including replicative and repair-error-associated mutagenesis, was examined.

Main Results:

• MPA significantly reduced the frequency of direct mutations induced by UV radiation (3.7-fold) and nitrous acid (33-fold).
• MPA exhibited protective effects against the lethal impact of UV radiation.
• MPA did not affect replicative mutagenesis but suppressed mutagenesis arising from DNA repair errors.

Conclusions:

• The findings suggest that MPA possesses notable antimutagenic activity.
• The observed antimutagenic effects of MPA are likely associated with the activation of post-replicative DNA repair pathways.