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DefinitionDiabetic retinopathy is a microvascular complication of diabetes affecting the retinal blood vessels.Risk FactorsDiabetic retinopathy is present in almost all individuals with type 1 diabetes and more than 60% of those with type 2 diabetes after two decades of disease.The risk increases with poor glycemic control, hypertension, dyslipidemia, smoking, pregnancy, and puberty.Although cataracts and glaucoma are also more frequent in people with diabetes, retinopathy remains the leading...
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Whole Vitreous Humor Dissection for Vitreodynamic Analysis
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Vitreous evaluation: a diagnostic challenge.

Manisha Mehta1, Reena A Rasheed2, Jay Duker3

  • 1Department of Pathology and Laboratory Medicine, Boston Medical Center, Boston, Massachusetts.

Ophthalmology
|December 3, 2014
PubMed
Summary
This summary is machine-generated.

This study analyzed 5736 vitreous samples, categorizing cytologic diagnoses and ancillary tests for vitrectomy. Effective communication between surgeons and pathologists is crucial for processing low-volume samples to ensure accurate diagnosis and timely treatment.

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Area of Science:

  • Ophthalmology
  • Cytopathology
  • Oncology

Background:

  • Vitrectomy is a surgical procedure to remove vitreous humor from the eye.
  • Cytologic diagnosis of vitreous samples aids in identifying intraocular diseases.
  • Appropriate processing of limited-volume vitreous samples is critical for accurate diagnosis.

Purpose of the Study:

  • To categorize vitrectomy cytologic diagnoses and ancillary tests.
  • To establish guidelines for processing low-volume vitreous samples.

Main Methods:

  • Retrospective case series of 5736 vitreous samples from three teaching institutions.
  • Review of cytologic diagnoses and processing protocols for diagnostic and therapeutic vitrectomies.
  • Categorization of diagnostic results (negative, suspicious, positive for malignancy) and documentation of ancillary studies.

Main Results:

  • Most samples (79.6%-99.89%) were negative for malignancy, with findings including vitreous hemorrhage, inflammation, and infection.
  • Ancillary studies included special stains, PCR for various pathogens (e.g., EBV, CMV), and cultures.
  • Malignant diagnoses (e.g., lymphoma, retinoblastoma, melanoma) were identified in a subset of cases, with specific ancillary tests employed.

Conclusions:

  • Cytologic evaluation of vitrectomy samples is valuable for differentiating malignancy, infections, and inflammatory conditions.
  • Effective preoperative communication between surgeons and pathologists is essential for selecting appropriate ancillary tests for small vitreous samples.
  • This study represents the largest dataset of cytologic diagnoses from vitrectomy samples.