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Related Experiment Video

Updated: Apr 20, 2026

Thrombus Profiling Assay: A Microfluidics-Based Platform for Comprehensively Characterizing Biomechanical Thrombogenesis
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A label-free and high sensitive aptamer biosensor based on hyperbranched polyester microspheres for thrombin

Chong Sun1, Qiaorong Han2, Daoying Wang3

  • 1Jiangsu Key Laboratory of Biofunctional Materials, Biomedical Functional Materials Collaborative Innovation Center, College of Chemistry and Materials Science, Nanjing Normal University, Nanjing 210023, China; Institute of Agricultural Products Processing, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China.

Analytica Chimica Acta
|December 3, 2014
PubMed
Summary

A novel label-free electrochemical aptamer biosensor was developed for thrombin detection in whole blood. This highly sensitive sensor utilizes hyperbranched polyester microspheres and a thrombin-binding aptamer for accurate measurement.

Keywords:
AntibiofoulingAptamer biosensorHyperbranched polyester microspheresThrombinWhole blood

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Area of Science:

  • Nanoscience and Biosensor Technology
  • Electrochemistry
  • Biomedical Diagnostics

Background:

  • Thrombin is a key biomarker in coagulation and various diseases.
  • Accurate and sensitive detection of thrombin is crucial for clinical diagnostics.
  • Existing detection methods often require complex procedures or labels.

Purpose of the Study:

  • To develop a label-free electrochemical aptamer biosensor for sensitive thrombin detection.
  • To utilize hyperbranched polyester microspheres for enhanced sensor performance.
  • To demonstrate the sensor's applicability in whole blood samples.

Main Methods:

  • Synthesis of carboxylic acid-functionalized hyperbranched polyester microspheres (HBPE-CA).
  • Modification of indium tin oxide (ITO) electrodes with HBPE-CA and subsequent grafting of thrombin-binding aptamer (TBA).
  • Electrochemical detection of thrombin based on the modulation of redox probe ([Fe(CN)6](3-/4-)) accessibility.

Main Results:

  • The aptamer biosensor exhibited high sensitivity and selectivity for thrombin.
  • A wide detection range from 10 fM to 100 nM was achieved.
  • A low detection limit of 0.90 fM was demonstrated in whole blood.
  • The biosensor showed good stability and repeatability, with no significant interference from BSA or lysozyme.

Conclusions:

  • The developed label-free electrochemical aptamer biosensor offers a promising platform for sensitive thrombin detection.
  • The integration of nanoscience and aptamer technology provides a cost-effective alternative to current methods.
  • This technology has potential applications in diagnostics and therapy for human health.