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Improved bioavailability of vitamin D3 using a β-lactoglobulin-based coagulum
Fatoumata Diarrassouba1, Ghislain Garrait2, Gabriel Remondetto3
1Chaire de recherche du Canada sur les protéines, les bio-systèmes et les aliments fonctionnels, Institut de recherche sur les nutraceutiques et les aliments fonctionnels (INAF/STELA), Université Laval, Québec, QC, Canada.
Food Chemistry
|December 3, 2014
Summary
Encapsulating Vitamin D3 (D3) in a beta-lactoglobulin matrix significantly enhances its stability and bioavailability. This novel approach improves D3
Area of Science:
- Food Science
- Biochemistry
- Nutritional Science
Background:
- Vitamin D3 (D3) is essential but faces stability challenges during storage and UV exposure.
- Encapsulation methods are explored to improve D3's solubility, stability, and bioavailability.
- Beta-lactoglobulin (βlg) is investigated as a potential matrix for D3 encapsulation.
Purpose of the Study:
- To encapsulate Vitamin D3 (D3) within a water-soluble beta-lactoglobulin (βlg) matrix.
- To evaluate the stability of encapsulated D3 under various conditions (cold storage, UV light, intestinal proteases).
- To determine the in vivo bioavailability of D3 from the βlg-based coagulum.
Main Methods:
- Formation of a βlg/D3 complex via acidification to create a water-soluble matrix.
- Assessment of D3 encapsulation efficiency.
- In vitro stability testing against UV light and intestinal proteases.
- In vivo bioavailability study in force-fed rats.
Main Results:
- High encapsulation efficiency (94.5 ± 1.8%) achieved for D3 within the βlg matrix.
- Significantly increased water solubility, long-term storage stability, and UV-light stability of D3 (p < 0.0001).
- Slow release of D3 due to protease resistance of the βlg matrix, enhancing D3 uptake and bioavailability in rats.
Conclusions:
- Acidification-induced βlg/D3 complexation effectively encapsulates D3 in a water-soluble matrix.
- The βlg matrix confers superior stability to D3 against environmental stressors.
- Encapsulated D3 exhibits enhanced bioavailability in vivo, demonstrating the potential of this delivery system.
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