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Antihypertensive effect of the bovine casein-derived peptide Met-Lys-Pro

Akio Yamada1, Takuma Sakurai1, Daisuke Ochi1

  • 1Morinaga Milk Industry Co., Ltd, Functional Food Research Department, Food Science & Technology Institute, 1-83, 5-Chome, Higashihara, Zama-City, Kanagawa-Pref. 252-8583, Japan.

Food Chemistry
|December 3, 2014
PubMed

Insights

Bovine casein peptide Met-Lys-Pro (MKP) effectively inhibits angiotensin I-converting enzyme (ACE). Oral administration of MKP lowers blood pressure in spontaneously hypertensive rats, suggesting its potential as an antihypertensive agent.

Area of Science:

  • Biochemistry
  • Pharmacology
  • Physiology

Background:

  • Hypertension remains a significant global health concern.
  • Dietary peptides are increasingly recognized for their potential health benefits.
  • Angiotensin I-converting enzyme (ACE) plays a crucial role in blood pressure regulation.

Purpose of the Study:

  • To investigate the antihypertensive effects of the bovine casein-derived peptide Met-Lys-Pro (MKP).
  • To evaluate the in vitro and in vivo efficacy of MKP as an ACE inhibitor.

Main Methods:

  • In vitro assessment of ACE-inhibitory activity.
  • In vivo pharmacokinetic study using radiolabeled MKP in spontaneously hypertensive rats (SHRs).
  • Measurement of thoracic aorta vasoconstriction.
  • Evaluation of systolic blood pressure (SBP) following single and repeated oral MKP administration in SHRs.

Main Results:

  • MKP demonstrated significant in vitro ACE-inhibitory activity (IC50 = 0.43 μM).
  • Orally administered MKP was absorbed into the plasma of SHRs.
  • MKP reduced angiotensin I-induced vasoconstriction in vitro.
  • Both single and repeated oral doses of MKP lowered SBP in SHRs.

Conclusions:

  • Orally administered MKP is absorbed and circulates in the plasma.
  • The ACE-inhibitory activity of MKP likely contributes to its observed antihypertensive effect.
  • MKP shows promise as a potential therapeutic agent for managing hypertension.
Keywords:
(14)C-labeled peptideACE-inhibitory peptideAngiotensin I-converting enzymeBioavailabilityCaseinHypertensionIle-Pro-Pro (CID 24978506)Val-Pro-Pro (CID 9818200)Val-Tyr (CID 4065033)

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