Related Experiment Video

Updated: Apr 20, 2026

Induction of Murine Intestinal Inflammation by Adoptive Transfer of Effector CD4+CD45RBhigh T Cells into Immunodeficient Mice
08:37

Induction of Murine Intestinal Inflammation by Adoptive Transfer of Effector CD4+CD45RBhigh T Cells into Immunodeficient Mice

Published on: April 21, 2015

17.9K

TRAIL receptor deletion in mice suppresses the inflammation of nutrient excess

Leila Idrissova1, Harmeet Malhi1, Nathan W Werneburg1

  • 1Division of Gastroenterology and Hepatology, Mayo Clinic, Rochester, MN 55905, United States.

Journal of Hepatology
|December 3, 2014
PubMed
Abstract

Insights

Tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) receptor signaling drives obesity-associated inflammation and metabolic dysfunction. Blocking this pathway prevents weight gain, insulin resistance, and liver damage in mice fed a high-fat diet.

Area of Science:

  • Immunology
  • Metabolic Syndrome Research
  • Molecular Biology

Background:

  • Low-grade chronic inflammation is a hallmark of metabolic syndrome.
  • The precise mechanisms driving obesity-associated inflammation remain unclear.

Purpose of the Study:

  • To investigate the role of TRAIL receptor (TR) signaling in the development of obesity-linked inflammation.
  • To utilize genetically modified mice lacking TR to study its function in metabolic disease.

Main Methods:

  • TR knockout (TR(-/-)) and wild-type (WT) mice were fed a high-fat, high-cholesterol, high-fructose (FFC) diet or standard chow.
  • Metabolic parameters, liver injury, and inflammation in liver and adipose tissues were evaluated.
  • Macrophage chemotaxis and activation were assessed in vitro.

Main Results:

  • TR deletion completely prevented weight gain, adiposity, and insulin resistance in FFC-fed mice.
  • TR(-/-) mice showed suppressed steatohepatitis, with normal liver enzymes and reduced fat accumulation.
  • Absence of TR inhibited macrophage accumulation and activation, reducing inflammation in the liver and adipose tissue.

Conclusions:

  • Macrophage-associated inflammation in the liver and adipose tissue, driven by nutrient excess, necessitates TR signaling.
  • Targeting TR signaling may offer a therapeutic strategy for metabolic syndrome.
Keywords:
LipoapoptosisMacrophage activationMetainflammationNon-alcoholic fatty liver disease

More Related Videos

Induction of Intestinal Inflammation by Adoptive Transfer of CBir1 TCR Transgenic CD4+ T Cells to Immunodeficient Mice07:34

Induction of Intestinal Inflammation by Adoptive Transfer of CBir1 TCR Transgenic CD4+ T Cells to Immunodeficient Mice

Published on: December 16, 2021

3.3K
Mechanistic Insight into the Development of TNBS-Mediated Intestinal Fibrosis and Evaluating the Inhibitory Effects of Rapamycin10:21

Mechanistic Insight into the Development of TNBS-Mediated Intestinal Fibrosis and Evaluating the Inhibitory Effects of Rapamycin

Published on: September 12, 2019

7.9K

Related Concept Videos

Inflammatory Response01:28

Inflammatory Response

An inflammatory response is a localized, nonspecific immune reaction that occurs when a tissue is injured. It is characterized by redness, swelling, heat, and pain, which are commonly called the cardinal signs and symptoms of inflammation. Inflammation can sometimes result in a loss of function.
Inflammation can be triggered by various stimuli, such as impact, abrasion, chemical irritation, infections, and extreme hot or cold temperatures. These can damage cells and connective tissue fibers,...
19.9K
Inflammatory Bowel Disease III: Crohn's Disease01:25

Inflammatory Bowel Disease III: Crohn's Disease

Crohn’s disease is a chronic, relapsing form of inflammatory bowel disease characterized by segmental, transmural inflammation that can affect any part of the gastrointestinal tract. Its pathogenesis arises from a combination of genetic susceptibility, environmental exposures, epithelial barrier dysfunction, and immune dysregulation. Together, these factors lead to an exaggerated immune response against components of the gut microbiome.Genetic and Environmental InfluencesMultiple genetic...
16
Receptor Downregulation in MVBs01:15

Receptor Downregulation in MVBs

Multivesicular bodies (MVBs) are mature endosomes that sort ubiquitinated proteins and then fuse with lysosomes to degrade the sorted proteins. Epidermal growth factor (EGF) and its receptor (EGFR) form a complex that can be internalized through endocytosis, sorted into an MVB, and later degraded.
The EGFR can initiate signaling pathways that  lead to cell proliferation, migration, and differentiation. Overexpression of EGFR  stimulates cells to proliferate. Excessive  EGFR...
3.0K
T Cell Types and Functions01:24

T Cell Types and Functions

When T cells with CD4 markers are activated, they give rise to two types of effector cells: helper T cells and regulatory T cells. Meanwhile, T cells with CD8 markers differentiate into effector cytotoxic T cells. The differentiation of CD4 T cells into helper T cell subsets, such as Th1, Th2, and Th17 cells, is dependent on the antigen type, antigen-presenting cell, and regulatory cytokines.
Th1 cells stimulate dendritic cells to express necessary co-stimulatory molecules on their surfaces for...
3.5K