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Updated: Apr 20, 2026
A Cell Culture Model for Producing High Titer Hepatitis E Virus Stocks
Published on: June 26, 2020
Oral immunization with recombinant hepatitis E virus antigen displayed on the Lactococcus lactis surface enhances
Shenyang Gao1, Dandan Li2, Ying Liu3
1Department of Animal Husbandry & Veterinary Medicine, Liaoning Medical University, No. 5-48 Renmin Street, Jinzhou 121001, China; Department of Food Science, Shenyang Agricultural University, No.120 Dongling Road, Shenyang 110161, China.
Abstract:
Hepatitis E virus (HEV) as a recognized zoonotic pathogen has posed global burden on public health, which is exacerbated by lack of efficient vaccine. In this study, we constructed a recombinant (inaQ-ORF2 gene fusion) Lactococcus lactis (L. lactis) strain NZ3900 that expresses and displays the hepatitis E virus antigen ORF2 utilizing an ice uncleation protein-based anchor system. After oral vaccination of BALB/c mice, significantly higher levels of ORF2-specific mucosal IgA and serum IgG were detected and cellular immunity was also induced. These findings further support that L. lactis-based HEV antigen vaccines could be used for human and animal protection against infection.
