Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Precipitate Formation and Particle Size Control

Precipitate Formation and Particle Size Control

In precipitation gravimetry, the precipitating agent should react specifically or selectively with the analyte. While a specific reagent reacts with the analyte alone, a selective reagent can react with a limited number of chemical species.
The obtained precipitate should be either a pure substance of known composition or easily converted to one by a simple process, such as ignition or drying. In addition, the precipitate should be insoluble and easily filterable. In general, filterability...

Pore Size Distribution

Pore Size Distribution

In concrete, the pore size distribution significantly influences the material's properties. Capillary pores, markedly larger than gel pores, form a vast network within partially hydrated cement paste, reducing the concrete's strength and increasing its permeability. This heightened permeability leads to a greater risk of damage from environmental factors like freeze-thaw cycles and chemical attacks, with the extent of vulnerability also being tied to the water-to-cement ratio.
Adequate...

Shape and Texture of Coarse Aggregate

Shape and Texture of Coarse Aggregate

Aggregate shape is classified based on the relative sharpness or roundness of the edges and corners. This classification includes categories like rounded, angular, elongated, and flaky, each with specific characteristics. Rounded aggregates, fully shaped by attrition, are typical of river or seashore gravel, while angular aggregates, such as crushed rock, have well-defined edges. Aggregates that are elongated and flaky are less desirable, as they can reduce the workability and strength of...

Basic Postulates of Kinetic Molecular Theory: Particle Size, Energy, and Collision

Basic Postulates of Kinetic Molecular Theory: Particle Size, Energy, and Collision

The ideal-gas equation, which is empirical, describes the behavior of gases by establishing relationships between their macroscopic properties. For example, Charles’ law states that volume and temperature are directly related. Gases, therefore, expand when heated at constant pressure. Although gas laws explain how the macroscopic properties change relative to one another, it does not explain the rationale behind it.

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

SEC-MALS analysis of polydeoxyribonucleotides: Revealing product heterogeneity and enabling measurement standardization.

Talanta·2026

Same author

Orthogonal Molecular Weight Analysis of Challenging Macromolecules Using MALS and Mass Photometry: Example of Polydeoxyribonucleotide.

Biomacromolecules·2026

Same author

Perioperative Care of Patients with Wearable Cardioverter Defibrillators.

Anesthesiology·2025

Same author

Studying host cell protein based on 2D nano-LC-MS/MS: comparison between the removal and the reservation of the main antibody components.

Analytical and bioanalytical chemistry·2025

Same author

Analysis of TEM micrographs with deep learning reveals APOE genotype-specific associations between HDL particle diameter and Alzheimer's dementia.

Cell reports methods·2025

Same author

Measuring the size of oil droplets in a flow cytometer using Mie resonances: A possible size calibration ladder for 0.5-6 μm.

Cytometry. Part A : the journal of the International Society for Analytical Cytology·2025

Search research articles

Related Experiment Video

Updated: Apr 20, 2026

Three-Dimensional Particle Shape Analysis Using X-ray Computed Tomography: Experimental Procedure and Analysis Algorithms for Metal Powders

Three-Dimensional Particle Shape Analysis Using X-ray Computed Tomography: Experimental Procedure and Analysis Algorithms for Metal Powders

Published on: December 4, 2020

Particle shape effects on subvisible particle sizing measurements.

Richard E Cavicchi¹, Michael J Carrier, Joshua B Cohen

¹Bioprocess Measurements Group, National Institute of Standards and Technology, Gaithersburg, Maryland.

Journal of Pharmaceutical Sciences

|December 3, 2014

Summary

Particle analysis instruments like flow imaging (FI) and electrical sensing zone (ESZ) show discrepancies with non-spherical particles. A new microfluidic device and algorithm improve accuracy for rod-shaped particle analysis.

Keywords:

electrical sensing zone flow imaging image analysis light obscuration light scattering (static)microscopy particle sizing physical characterization

More Related Videos

Measurement of Particle Size Distribution in Turbid Solutions by Dynamic Light Scattering Microscopy

Measurement of Particle Size Distribution in Turbid Solutions by Dynamic Light Scattering Microscopy

Published on: January 9, 2017

Fabrication and Characterization of Thickness Mode Piezoelectric Devices for Atomization and Acoustofluidics

Fabrication and Characterization of Thickness Mode Piezoelectric Devices for Atomization and Acoustofluidics

Published on: August 5, 2020

Related Experiment Videos

Last Updated: Apr 20, 2026

Three-Dimensional Particle Shape Analysis Using X-ray Computed Tomography: Experimental Procedure and Analysis Algorithms for Metal Powders

Three-Dimensional Particle Shape Analysis Using X-ray Computed Tomography: Experimental Procedure and Analysis Algorithms for Metal Powders

Published on: December 4, 2020

Measurement of Particle Size Distribution in Turbid Solutions by Dynamic Light Scattering Microscopy

Measurement of Particle Size Distribution in Turbid Solutions by Dynamic Light Scattering Microscopy

Published on: January 9, 2017

Fabrication and Characterization of Thickness Mode Piezoelectric Devices for Atomization and Acoustofluidics

Fabrication and Characterization of Thickness Mode Piezoelectric Devices for Atomization and Acoustofluidics

Published on: August 5, 2020

Area of Science:

Analytical Chemistry
Materials Science
Biotechnology

Background:

Subvisible particle analysis (<100 μm) using light obscuration, flow imaging (FI), and electrical sensing zone (ESZ) shows discrepancies, especially with non-spherical particles.
Photolithography was used to create rod and disk-shaped particles to investigate shape effects on instrument performance.

Purpose of the Study:

To elucidate the causes of discrepancies and peak broadening in particle analysis instruments when characterizing non-spherical particles.
To develop and validate an improved correction algorithm for flow imaging (FI) to enhance accuracy for rod-shaped particles.

Main Methods:

Fabrication of rod and disk-shaped particles using photolithography.
Development of a microfluidic device for simultaneous electrical sensing zone (ESZ) and flow imaging (FI) measurements.
Implementation of an improved correction algorithm for FI to account for diffraction and focus effects.

Main Results:

Instruments produced broadened peaks and differing mean size parameters for monodisperse rod-shaped particles.
Particle alignment with flow caused oversizing by FI and undersizing by ESZ.
Observed particle tumbling in the microfluidic device led to oversizing and broader size distributions for rods.

Conclusions:

Particle shape significantly impacts subvisible particle analysis, causing discrepancies between different instruments.
The developed microfluidic device and improved FI algorithm reduce measurement discrepancies for rod-shaped particles.
Understanding particle orientation and tumbling is crucial for accurate subvisible particle characterization.