Related Experiment Videos
Persistent replication of herpes simplex virus type 1 in JOK-1 cells
K Thiele1, S Mittnacht, H Kirchner
1Institute for Virus Research, German Cancer Research Center, Heidelberg, F.R.G.
The Journal of General Virology
|July 1, 1989
Summary
Herpes simplex virus type 1 infection persisted in human B cells for over a year. The cells restricted viral replication, suggesting an unknown intrinsic cellular property limits herpes simplex virus type 1 spread.
Area of Science:
- Virology
- Immunology
- Cell Biology
Background:
- Herpes simplex virus type 1 (HSV-1) establishes persistent infections in humans.
- Understanding the mechanisms of viral persistence and host cell interaction is crucial.
Purpose of the Study:
- To investigate the long-term effects of HSV-1 infection on the human B cell line JOK-1.
- To elucidate the mechanisms underlying restricted viral replication in infected cells.
Main Methods:
- Infection of JOK-1 cells with HSV-1.
- Infectious centre assays to quantify infectious virus production.
- Immunofluorescence to detect viral proteins (ICP4, glycoproteins).
- In situ hybridization to detect viral DNA.
Main Results:
- HSV-1 infection persisted in JOK-1 cells for over 12 months without culture extinction.
- A small percentage of cells produced infectious virus, but a larger proportion contained viral proteins and DNA.
- No evidence of interferon involvement in restricting viral replication was found.
Conclusions:
- JOK-1 cells support persistent HSV-1 infection with restricted viral replication and maturation.
- An intrinsic cellular property, independent of interferon, likely mediates this restriction.
- Further research is needed to identify the specific cellular mechanisms involved.