Related Experiment Videos
Characterization of two genome types of adenovirus type 31 isolated in Stockholm during 1987
M E Johansson1, M Brundin, L Adamson
1Department of Clinical Microbiology, Karolinska Hospital, Stockholm, Sweden.
Abstract:
The authors isolated during 1987 seven adenovirus type 31 (Ad31) within a 9-month period. The isolates were obtained from urine, throat, and feces, implying a systemic spread of the infection. Most patients displayed gastrointestinal symptoms, but some had respiratory symptoms and fever. All of the strains differed by restriction endonuclease analysis from the prototype strain (1315) by an additional Bgl II restriction site. Ad31 isolates 1-6 could be divided into two groups by the enzymes Bam HI, Msp I, and Xho I. Each enzyme gave rise to the same group distribution: isolates 1-3 and 4-6, respectively. Digestion with Bst EII, Hind III, Kpn I, and Sma I resulted in identical patterns for isolates 1-6. Isolate 7, however, demonstrated a DNA deletion of approximately 0.8 kbp, but it was otherwise identical to isolates 4-6. In conclusion, two separate genome types of Ad31 were isolated, one of which included a DNA deletion mutant. The increased isolation rate may reflect an epidemiological situation, as the same isolation procedure had been used both before and after this period.
Insights
Seven adenovirus type 31 (Ad31) strains were isolated in 1987, showing systemic spread and two distinct genome types. One Ad31 isolate presented a DNA deletion, suggesting an evolving viral population.
Area of Science:
- Virology
- Molecular Biology
- Infectious Diseases
Background:
- Adenovirus type 31 (Ad31) infections can manifest with diverse symptoms, including gastrointestinal and respiratory issues.
- Systemic spread of Ad31 is suggested by isolation from urine, throat, and feces.
- Understanding Ad31 genomic diversity is crucial for tracking its epidemiology.
Purpose of the Study:
- To characterize the genomic profiles of Ad31 isolates obtained during an increased isolation period.
- To investigate potential genomic variations within Ad31 strains.
Main Methods:
- Restriction endonuclease analysis was employed to compare Ad31 isolates against the prototype strain.
- Multiple enzymes including Bgl II, Bam HI, Msp I, Xho I, Bst EII, Hind III, Kpn I, and Sma I were used for DNA digestion.
- DNA fragment sizes were analyzed to identify variations and deletions.
Main Results:
- All seven Ad31 isolates exhibited an additional Bgl II restriction site compared to the prototype strain.
- Isolates 1-6 were classified into two groups based on Bam HI, Msp I, and Xho I digestion patterns.
- Isolate 7 displayed a DNA deletion of approximately 0.8 kbp, while being otherwise similar to isolates 4-6.
Conclusions:
- Two distinct Ad31 genome types were identified during the study period.
- The isolation of a DNA deletion mutant suggests genomic plasticity within Ad31.
- The increased isolation rate may indicate a specific epidemiological event or increased prevalence of Ad31.