Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Phosphoinositides and PIPs01:42

Phosphoinositides and PIPs

11.8K
Phosphoinositides are a group of phospholipids containing a glycerol backbone with two fatty acid chains and a phosphate attached to a myoinositol sugar ring. The inositol head group extends into the cytoplasm, where it is modified by adding phosphate groups to form phosphatidylinositol phosphates or PIPs.
Different phosphoinositides are synthesized and recruited on the cytosolic face of the plasma membrane. The localization of specific phosphoinositides concentrated in separate membrane...
11.8K
Pulmonary Hypertension: Classification and Pathogenesis01:30

Pulmonary Hypertension: Classification and Pathogenesis

906
Pulmonary hypertension (PH) is a severe health condition in which the mean pulmonary arterial pressure increases to 25 mmHg or more, even when the body is at rest. This high pressure in the blood vessels that transport blood from the heart to the lungs can cause various symptoms, including shortness of breath, can lead to right heart failure, and significantly affect the overall quality of life.
There are various classifications for PH, each relating to different underlying causes and also...
906
Asymmetric Lipid Bilayer01:35

Asymmetric Lipid Bilayer

11.2K
Biological membranes show uneven distribution of different types of lipids in the inner and outer layers, resulting in transverse asymmetric membranes. The treatment of the erythrocyte membrane with the enzyme phospholipase confirmed the asymmetric nature of the lipid bilayer. The enzyme hydrolyzes lipids into fatty acids and hydrophilic groups. The phospholipase acts only on the outer layer of the membrane, while the inner layer remains intact. The phospholipase treatment resulted in 80%...
11.2K
Pulmonary Edema II: Pathophysiology01:18

Pulmonary Edema II: Pathophysiology

16
Pulmonary edema is the accumulation of fluid in the interstitial and alveolar spaces of the lungs, impairing gas exchange and oxygen delivery. It may be cardiogenic or noncardiogenic, but both reduce oxygenation and lung compliance.Cardiogenic Pulmonary EdemaCardiogenic edema results from increased hydrostatic pressure in pulmonary capillaries, usually due to left ventricular dysfunction from myocardial infarction, heart failure, or valvular disease. Ineffective cardiac pumping causes blood to...
16
Cirrhosis II: Pathophysiology01:24

Cirrhosis II: Pathophysiology

24
Cirrhosis is a progressive chronic liver injury caused by prolonged inflammation, excessive fibrotic remodeling, and impaired regeneration. Over time, repeated hepatic insults disrupt the liver’s architecture and function, leading to reduced blood flow, impaired bile drainage, and diminished metabolic capacity.Pathophysiology of cirrhosisCirrhosis arises from three main responses to chronic liver damage: inflammation, immune activation, and hepatocyte death. These processes lead to...
24
Cystic Fibrosis: Pathogenesis01:23

Cystic Fibrosis: Pathogenesis

1.1K
Cystic fibrosis (CF), an autosomal recessive disorder, significantly affects the function of exocrine glands. This genetically inherited disease is characterized by the production of thick and sticky mucus, which can severely affect various organs and systems in the body.
CF is primarily caused by a genetic mutation in a chromosome 7 gene coding for the cystic fibrosis transmembrane conductance regulator (CFTR) protein. The most common gene mutation leading to CF is the ΔF508 mutation,...
1.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Hemoglobin's α-Helix-to-β-Sheet Transition Enables Targeted mRNA Delivery to the Lung.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2026
Same author

GSK2879552 inhibits NLRP3 inflammasome-mediated pyroptosis and acute lung injury through NOX2-ROS signalling axis.

Biochemical pharmacology·2026
Same author

FTIR imaging identifies alterations in lung tissue structure and biochemical composition in human idiopathic pulmonary fibrosis.

Scientific reports·2026
Same author

CaMKKβ regulates transcription factor Elf2 gene methylation to maintain endothelial junctional barrier integrity.

iScience·2026
Same author

Rosarin alleviates dry eye disease via suppressing NF-κB signal mediated inflammation and M1 polarization in macrophage.

European journal of pharmacology·2025
Same author

The cGAS-STING Pathway in Pulmonary Diseases: Mechanisms and Therapeutic Potential.

International journal of molecular sciences·2025

Related Experiment Video

Updated: Apr 20, 2026

Oropharyngeal Administration of Bleomycin in the Murine Model of Pulmonary Fibrosis
06:03

Oropharyngeal Administration of Bleomycin in the Murine Model of Pulmonary Fibrosis

Published on: May 9, 2025

2.4K

Sphingolipids in pulmonary fibrosis.

Long Shuang Huang1, Viswanathan Natarajan2

  • 1Department of Pharmacology, University of Illinois at Chicago, IL, USA.

Advances in Biological Regulation
|December 3, 2014
PubMed
Summary

Sphingosine kinase 1 (SphK1) and S1P lyase (S1PL) are key players in lung fibrosis. Targeting SphK1 may offer a new therapeutic strategy for idiopathic pulmonary fibrosis (IPF).

Keywords:
AutophagyPulmonary fibrosisS1P lyaseSphingosine kinase 1Sphingosine-1-phosphateTGF-β

More Related Videos

Refined Murine Model of Idiopathic Pulmonary Fibrosis
07:51

Refined Murine Model of Idiopathic Pulmonary Fibrosis

Published on: June 17, 2025

1.4K
A Mouse Model of Pulmonary Fibrosis Induced by Nasal Bleomycin Nebulization
02:46

A Mouse Model of Pulmonary Fibrosis Induced by Nasal Bleomycin Nebulization

Published on: January 20, 2023

5.2K

Related Experiment Videos

Last Updated: Apr 20, 2026

Oropharyngeal Administration of Bleomycin in the Murine Model of Pulmonary Fibrosis
06:03

Oropharyngeal Administration of Bleomycin in the Murine Model of Pulmonary Fibrosis

Published on: May 9, 2025

2.4K
Refined Murine Model of Idiopathic Pulmonary Fibrosis
07:51

Refined Murine Model of Idiopathic Pulmonary Fibrosis

Published on: June 17, 2025

1.4K
A Mouse Model of Pulmonary Fibrosis Induced by Nasal Bleomycin Nebulization
02:46

A Mouse Model of Pulmonary Fibrosis Induced by Nasal Bleomycin Nebulization

Published on: January 20, 2023

5.2K

Area of Science:

  • Pulmonary Medicine
  • Biochemistry
  • Cell Biology

Background:

  • Idiopathic pulmonary fibrosis (IPF) involves lung tissue damage and extracellular matrix deposition.
  • Elevated sphingosine-1-phosphate (S1P) levels are observed in IPF patients and animal models.
  • The precise role of S1P metabolism in pulmonary fibrosis pathogenesis is not fully understood.

Purpose of the Study:

  • To investigate the in vivo role of S1P metabolism in pulmonary fibrosis.
  • To evaluate sphingosine kinases (SphKs) and S1P lyase (S1PL) as potential therapeutic targets for IPF.

Main Methods:

  • Microarray analysis of blood cells from IPF patients.
  • Utilized SphK1, SphK2, and S1PL knockout mouse models.
  • Employed SphK inhibitor and bleomycin-induced pulmonary fibrosis model.

Main Results:

  • SphK1 expression negatively correlated with lung function and survival in IPF patients.
  • SphK1 and S1PL expression increased in IPF lung tissues and bleomycin-challenged mice.
  • SphK1 knockdown or inhibition attenuated fibrosis; S1PL deletion potentiated it.
  • SphK1 inhibition reduced mortality and fibrosis in a bleomycin model.

Conclusions:

  • SphK1 and S1PL are critical in lung fibrosis pathology.
  • Targeting SphK1 presents a promising therapeutic avenue for pulmonary fibrosis.
  • S1PL's role in regulating fibroblast differentiation warrants further investigation.