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Related Experiment Video

Updated: Apr 20, 2026

Isolation of Salmonella typhimurium-containing Phagosomes from Macrophages

Isolation of Salmonella typhimurium-containing Phagosomes from Macrophages

Published on: October 25, 2017

Isolating phagosomes from tissue culture cells.

Paul R Pryor¹, Adam P Rofe¹

¹Centre for Immunology and Infection, Hull York Medical School and Department of Biology, University of York, York YO10 5DD, United Kingdom.

Cold Spring Harbor Protocols

|December 3, 2014

Summary

This summary is machine-generated.

This study presents a new, rapid method for isolating phagosomes using magnetic beads, bypassing difficult centrifugation techniques. This approach simplifies the study of phagocytosis in tissue culture cells.

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A Simple and Efficient Method to Isolate Macrophages from Mixed Primary Cultures of Adult Liver Cells

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A Simple and Efficient Method to Isolate Macrophages from Mixed Primary Cultures of Adult Liver Cells

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Published on: May 24, 2011

Area of Science:

Cell Biology
Immunology

Background:

Phagocytosis is a critical cellular process involving the engulfment of particles.
Isolation of phagosomes is essential for studying cellular processes.
Current isolation methods using centrifugation are time-consuming and complex.

Purpose of the Study:

To develop a rapid and simplified protocol for isolating phagosomes.
To overcome the limitations of existing centrifugation-based methods.

Main Methods:

Utilized magnetic beads for particle uptake by tissue culture cells.
Developed a protocol that avoids centrifugation for phagosome isolation.

Main Results:

Successfully isolated the phagosomal compartment rapidly.
The new method is significantly faster and less labor-intensive than traditional techniques.

Conclusions:

The magnetic bead-based protocol offers an efficient alternative for phagosome isolation.
This method facilitates the study of phagocytosis and related cellular mechanisms.