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Intact cell mass spectrometry as a progress tracking tool for batch and fed-batch fermentation processes.

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  • 1Institute of Chemical Technologies and Analytics, Vienna University of Technology, A-1060 Vienna, Austria.

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Summary

Intact cell mass spectrometry (ICMS) offers a rapid method for monitoring Penicillium chrysogenum fermentation. This technique successfully differentiates fungal growth stages, paving the way for improved penicillin production processes.

Keywords:
BatchFed-batchFermentationIntact cell mass spectrometryPenicillin VPenicillium chrysogenum

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Area of Science:

  • Biotechnology
  • Microbiology
  • Analytical Chemistry

Background:

  • Penicillin production by Penicillium chrysogenum is a long-standing research area.
  • Monitoring fermentation progress is crucial for optimizing antibiotic yields.
  • Existing methods for fermentation monitoring can be time-consuming.

Purpose of the Study:

  • To evaluate Intact Cell Mass Spectrometry (ICMS) as a rapid monitoring tool for Penicillium chrysogenum fermentation.
  • To optimize the ICMS method for penicillin V fermentation broth samples.
  • To demonstrate the applicability of ICMS for differentiating fungal growth stages.

Main Methods:

  • Optimization of the ICMS method for fermentation broth samples.
  • Analysis of ICMS data using hierarchical cluster analysis and specialized software.
  • Application of matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) instruments.

Main Results:

  • ICMS successfully monitored penicillin V production in real-time.
  • Hierarchical cluster analysis differentiated batch and fed-batch fermentation stages.
  • The ICMS method showed universal applicability across different MALDI-TOF instruments.

Conclusions:

  • ICMS is a fast and effective method for monitoring Penicillium chrysogenum fermentation.
  • The developed ICMS method provides valuable insights into fungal growth stages.
  • This technique offers a foundation for a user-friendly fermentation monitoring system.