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Variation in herpes simplex virus type 1 glycoproteins produced in kidney cells from different species
M N Al-Ahdal1, F A Al-Khodairy, T J McGarry
1Department of Biological and Medical Research, King Faisal Specialist Hospital and Research Centre, Riyadh, Saudia Arabia.
Abstract:
Viral glycoproteins from herpes simplex virus, type 1 (HSV-1) infected NBL-1, Vero, and BHK-21 cells were labelled with 14C-glucosamine and studied by SDS-PAGE and Con-A chromatography. SDS-PAGE analysis demonstrated differences in the number and molecular weight of glycoproteins from these cells. Con-A chromatography resulted in similar binding of glycoproteins from NBL-1 and Vero cells of 10.5 and 18.6%, respectively, whereas BHK-21 cells showed binding of 65%. These studies indicate that HSV-1 glycoprotein oligosaccharide processing is variable in kidney cells of different species.