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Morphological characterization of mammalian timeless in the mouse brain development
Yutaka Inaguma1, Hidenori Ito1, Akira Hara2
1Department of Molecular Neurobiology, Institute for Developmental Research, Aichi Human Service Center, Aichi 480-0392, Japan.
Neuroscience Research
|December 3, 2014
Summary
Timeless (Tim) protein expression and localization change during mouse brain development. Tim is found in neurons, with its location shifting from the nucleus in developing brains to the cytoplasm in adults.
Area of Science:
- Neuroscience
- Developmental Biology
- Molecular Biology
Background:
- Timeless (Tim) is crucial for circadian rhythm in Drosophila.
- Mammalian Tim is involved in cell cycle control and embryonic development.
Purpose of the Study:
- To analyze the expression and localization of Tim during mouse brain development.
- To understand Tim's role in neuronal development.
Main Methods:
- Generated a specific antibody against Tim.
- Performed Western blotting, immunohistochemistry, and in situ hybridization.
- Analyzed primary cultured mouse hippocampal neurons.
Main Results:
- Tim protein is detected throughout mouse brain development.
- Tim expression is prominent in developing neurons (ventricular/subventricular zones, cortex).
- Tim localization shifts from the nucleus (embryonic/early postnatal) to the cytoplasm (adult) in cortical and hippocampal neurons.
Conclusions:
- Tim exhibits spatiotemporal regulation in neuronal tissues.
- Tim likely plays developmental stage-specific roles in neuronal functions.
