Related Experiment Video

Updated: Apr 20, 2026

Methods to Study Mrp4-containing Macromolecular Complexes in the Regulation of Fibroblast Migration
10:43

Methods to Study Mrp4-containing Macromolecular Complexes in the Regulation of Fibroblast Migration

Published on: May 19, 2016

7.1K

FERM domain-containing protein FRMD5 regulates cell motility via binding to integrin β5 subunit and ROCK1

Jinxia Hu1, Miaomiao Niu2, Xueying Li2

  • 1Key Laboratory of Carcinogenesis and Translational Research, Ministry of Education, Peking University Health Science Center, Beijing 100191, China; State Key Laboratory of Natural and Biomimetic Drugs, Peking University Health Science Center, Beijing 100191, China; Laboratory of Molecular Cell Biology and Tumor Biology, Department of Anatomy, Histology and Embryology, Peking University Health Science Center, Beijing 100191, China; Department of Biochemistry and Molecular Biology, Peking University Health Science Center, Beijing 100191, China; Department of Biochemistry and Molecular Biology, Binzhou Medical University, Yantai 264000, Shandong, China.

FEBS Letters
|December 3, 2014
PubMed

Insights

FRMD5 protein inhibits lung cancer cell migration by promoting adhesion and inhibiting ROCK1 activation. This dual mechanism involves binding to integrin β5 and ROCK1, revealing FRMD5

Area of Science:

  • Oncology
  • Cell Biology
  • Molecular Biology

Background:

  • FRMD5 (FERM domain-containing protein 5) is implicated in tumor progression.
  • The precise mechanisms by which FRMD5 inhibits cancer cell migration remain unclear.

Purpose of the Study:

  • To elucidate the molecular mechanisms of FRMD5-mediated inhibition of cell migration in human lung cancer.
  • To investigate the interactions of FRMD5 with key cellular components involved in cell motility.

Main Methods:

  • Investigated FRMD5 interactions with integrin β5 cytoplasmic tail and ROCK1 in lung cancer cells.
  • Assessed the effects of FRMD5 on cell-matrix adhesion, cell spreading, and cell migration.
  • Analyzed ROCK1 activation, myosin light chain phosphorylation, and actin stress fiber formation.

Main Results:

  • FRMD5 interacts with integrin β5 and ROCK1, inhibiting lung cancer cell migration.
  • FRMD5 enhances cell-matrix adhesion and cell spreading on vitronectin.
  • FRMD5 binding to ROCK1 suppresses its activation, leading to reduced myosin light chain phosphorylation and actin stress fiber formation.

Conclusions:

  • FRMD5 acts as a tumor suppressor by inhibiting cell motility through a dual pathway.
  • FRMD5 regulates tumor cell migration via interactions with integrin β5 and ROCK1.
Keywords:
Cell migrationFRMD5Integrin β5ROCK1

More Related Videos

Analyzing Cell Surface Adhesion Remodeling in Response to Mechanical Tension Using Magnetic Beads07:55

Analyzing Cell Surface Adhesion Remodeling in Response to Mechanical Tension Using Magnetic Beads

Published on: March 8, 2017

9.0K
Spatio-Temporal Manipulation of Small GTPase Activity at Subcellular Level and on Timescale of Seconds in Living Cells10:27

Spatio-Temporal Manipulation of Small GTPase Activity at Subcellular Level and on Timescale of Seconds in Living Cells

Published on: March 9, 2012

11.3K

Related Concept Videos

Intracellular Signaling Affects Focal Adhesions01:17

Intracellular Signaling Affects Focal Adhesions

Integrins act both as extracellular input receivers and as intracellular processing activators. As their name suggests, integrins are entirely integrated into the membrane structure. Their hydrophobic membrane-spanning regions interact with the phospholipid bilayer's hydrophobic region. These membrane receptors provide extracellular attachment sites for effectors like hormones and growth factors. They activate intracellular response cascades when their effectors are bound and active.
Some...
3.9K
Integrins01:10

Integrins

Animal and protozoan cells do not have cell walls to help maintain shape and provide structural stability. Instead, these eukaryotic cells secrete a sticky mass of carbohydrates and proteins into the spaces between adjacent cells. This network of proteins and molecules is called an extracellular matrix or ECM.
Some ECM proteins assemble into a basement membrane to which the remaining components adhere. Proteoglycans typically form the bulk of the ECM while fibrous proteins, like collagen,...
6.3K
Activation of Integrins01:15

Activation of Integrins

Integrins bind ligands and transmit information from outside the cell to inside or vice-versa through an "outside-in signaling" or "inside-out signaling."
In "outside-in signaling," external factors in the extracellular space bind to exposed ligand binding sites on integrins. This causes the inactive protein to undergo a conformational change to become active. Integrins are often clustered on the cell membrane. Repetitive and regularly spaced ligand binding...
5.8K
Cytoskeletal Coordination in Cell Migration01:32

Cytoskeletal Coordination in Cell Migration

A migrating cell changes its shape during the cyclic events of attachment and detachment from the substratum and repositions the cell organelles correspondingly. These complex events are orchestrated by the dynamic cytoskeletal network comprising actin filaments, intermediate filaments, and microtubules. Cytoskeletal crosstalk — the direct and indirect communication between the different components — is crucial for this coordination. Direct communication involves various linker...
5.8K
Cell Motility through Blebbing01:16

Cell Motility through Blebbing

Blebs are a type of membrane protrusion formed by the internal hydrostatic pressure of the cytoplasm. Blebs are observed in several cell types, including fibroblasts, immune cells, and single-celled organisms like the amoeba. The primary function of blebs is cell locomotion and apoptosis, but they are also found during necrosis and cell division. The life cycle of a bleb comprises an initiation phase followed by the expansion and retraction phases.
Blebbing Through the Matrix
In multicellular...
2.8K
Cell Polarization by Rho Proteins01:21

Cell Polarization by Rho Proteins

Cell polarity is the asymmetric distribution of cellular and membrane components, making one side of the cell different from the other. This polarity is essential to many processes such as embryogenesis, axon migration, glucose transport across epithelial cells, and directional cell migration. A migrating cell responds to intracellular or extracellular signals via molecular cascades that reorganize the actin cytoskeleton to establish this polarity. In these cells, the Rho family proteins Cdc42,...
4.2K