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Dissecting high from low responders in a vitamin D3 intervention study
Noora Saksa1, Antonio Neme1, Jussi Ryynänen1
1School of Medicine, Institute of Biomedicine, University of Eastern Finland, P.O. Box 1627, FIN-70211 Kuopio, Finland.
The Journal of Steroid Biochemistry and Molecular Biology
|December 3, 2014
Summary
This study identified key gene expression and clinical markers to distinguish vitamin D3 responders in pre-diabetic individuals. These biomarkers help understand vitamin D
Area of Science:
- Endocrinology and Metabolism
- Molecular Biology
- Genomics
Background:
- Vitamin D3 is a crucial signaling molecule influencing numerous genes via the vitamin D receptor (VDR).
- Identifying individuals who respond effectively to vitamin D3 supplementation is vital for personalized medicine.
- Pre-diabetic individuals represent a key population for studying metabolic responses to vitamin D.
Purpose of the Study:
- To identify transcriptomic and clinical biomarkers for classifying vitamin D3 responders in pre-diabetic subjects.
- To correlate changes in VDR target gene expression in PBMCs with serum 25(OH)D3 levels.
- To assess the utility of various biochemical and clinical parameters in reflecting vitamin D3's pleiotropic effects.
Main Methods:
- Analysis of 12 known VDR target genes in peripheral blood mononuclear cells (PBMCs) from 71 pre-diabetic subjects.
- Correlation of gene expression changes with serum 25-hydroxyvitamin D3 (25(OH)D3) levels before and after a 5-month intervention.
- Systematic assessment of 12 biochemical and clinical parameters for their correlation with 25(OH)D3 levels.
Main Results:
- 55-62% of participants were classified as significant vitamin D3 responders based on gene expression.
- Gene expression changes in PBMCs correlated significantly with serum 25(OH)D3 levels.
- 12 biochemical/clinical parameters, including parathyroid hormone (PTH) and insulin, showed correlations with 25(OH)D3 comparable to VDR target genes.
Conclusions:
- Transcriptomic and serum biomarkers effectively describe vitamin D3-induced changes in PBMCs.
- These biomarkers allow for the segregation of individuals into high and low vitamin D3 responders.
- A network of responses exists, with PTH playing a central role in vitamin D3's pleiotropic effects.
Keywords:
Correlation analysisGene regulationPrimary vitamin D target genesVitamin D receptorVitamin D(3)Vitamin D(3) supplementationMore Related Videos
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