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STRait Razor v2.0: the improved STR Allele Identification Tool--Razor
David H Warshauer1, Jonathan L King1, Bruce Budowle2
1Institute of Applied Genetics, Department of Molecular and Medical Genetics, University of North Texas Health Science Center, 3500 Camp Bowie Boulevard, Fort Worth, TX 76107, USA.
Forensic Science International. Genetics
|December 3, 2014
Summary
STRait Razor v2.0 is a bioinformatic tool for identifying short tandem repeat (STR) alleles in sequencing data. This updated software offers improved locus detection and genotyping, ensuring reliable allele calls comparable to traditional methods.
Area of Science:
- Bioinformatics
- Genetics
- Forensic Science
Background:
- Short tandem repeats (STRs) are crucial genetic markers used in human identification.
- Massively parallel sequencing (MPS) offers high-throughput data generation for genetic analysis.
- Accurate STR allele identification from MPS data is essential for forensic and genetic studies.
Purpose of the Study:
- To introduce STRait Razor v2.0, an enhanced bioinformatic tool for STR allele identification.
- To improve upon the original STRait Razor software with new features and expanded capabilities.
- To provide a reliable method for STR genotyping from MPS raw data.
Main Methods:
- STRait Razor v2.0 utilizes a detection method for reliable STR allele calling.
- The software incorporates an expanded locus configuration file, including X-chromosome STRs and Amelogenin.
- Features include a custom locus list generator, novel output sorting for variation detection, and an electropherogram-emulating genotyping tool.
Main Results:
- STRait Razor v2.0 successfully detects a wider range of STR loci compared to the previous version.
- The software provides user options for targeted STR analysis (autosomal, X, Y, or all).
- Concordance testing confirmed complete consistency between STRait Razor v2.0 and the original software's allele calls.
Conclusions:
- STRait Razor v2.0 is a robust and versatile tool for STR allele identification from MPS data.
- The enhancements in v2.0 increase its utility for various genetic applications, including forensic analysis.
- The software ensures reliable and consistent genotyping results, comparable to established methods like capillary electrophoresis.
