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Author Spotlight: Investigating Wolbachia-Induced Thelytokous Parthenogenesis and Genetic Toolkit Development Through RNA Interference
Published on: November 21, 2023
Construction of an efficient RNAi system in the cellulolytic fungus Trichoderma reesei
Ronglin He1, Wei Guo2, Lixian Wang2
1Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, 32 West 7th Avenue, Tianjin Airport Economic Area, Tianjin 300308, PR China; Key Laboratory of Industrial Fermentation Microbiology (Tianjin University of Science & Technology), Ministry of Education, Tianjin 300457, PR China; Tianjin Key Lab of Industrial Microbiology, Tianjin University of Science and Technology, Tianjin 300457, PR China.
Abstract:
An improved RNA interference method was developed in Trichoderma reesei, using convergent dual promoters for efficient and high-throughput RNA silencing. This new vector allowed for the silencing of the eGFP gene and target genes to occur simultaneously, significantly facilitating the rapid screening of the transformants using eGFP as a reporter.
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