[Immobilized oligonucleotides as affinity sorbents for restriction endonucleases]

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Microfluidic On-chip Capture-cycloaddition Reaction to Reversibly Immobilize Small Molecules or Multi-component Structures for Biosensor Applications
14:43

Microfluidic On-chip Capture-cycloaddition Reaction to Reversibly Immobilize Small Molecules or Multi-component Structures for Biosensor Applications

Published on: September 23, 2013

S Kh Degtiarev, P A Belavin, I G Shishkina

    Bioorganicheskaia Khimiia
    |March 1, 1989
    PubMed

    Abstract:

    Affinity chromatography of IIS type restriction endonucleases is proposed. It is shown that endonucleases HgaI, FokI, and SfaNI have affinity to the matrix with immobilized oligonucleotides which contain the endonuclease's recognition sites resistant to the hydrolysis.

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    Restriction Enzymes01:11

    Restriction Enzymes

    Restriction enzymes are bacterial enzymes used to cut DNA in a sequence-specific manner. To cleave DNA, they bind to specific palindromic sequences called restriction sites. Such palindromic DNA sequences or inverted repeats are commonly found in regions of functional significance, such as the origin of replication, gene operator sites, and regions containing transcription termination signals.
    The host bacteria protect their own genomic DNA from these enzymes by methylating these sites. Some...

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