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Updated: Apr 20, 2026

A Simple and Rapid Protocol for Measuring Neutral Lipids in Algal Cells Using Fluorescence

A Simple and Rapid Protocol for Measuring Neutral Lipids in Algal Cells Using Fluorescence

Published on: May 30, 2014

Rapid method to screen and sort lipid accumulating microalgae.

Iago Teles Dominguez Cabanelas¹, Mathijs van der Zwart², Dorinde M M Kleinegris³

¹Wageningen University, Bioprocess Engineering, AlgaePARC, P.O. Box 8128, 6700 EV Wageningen, Netherlands; Wageningen University and Research Center, Food and Biobased Research, AlgaePARC, Bornsesteeg 10, Building 112, 6721NG Bennekom, Netherlands.

Bioresource Technology

|December 3, 2014

Summary

This study presents an efficient fluorescence activated cell sorting (FACS) staining method for Chlorococcum littorale. The optimized method uses BODIPY505/515 to detect and sort high-lipid cells while maintaining viability.

Keywords:

BODIPY(505/515)Chlorococcum littorale FACS Lipids Microalgae

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Last Updated: Apr 20, 2026

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Published on: May 30, 2014

Cultivation of Green Microalgae in Bubble Column Photobioreactors and an Assay for Neutral Lipids

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Analysis of Fatty Acid Content and Composition in Microalgae

Analysis of Fatty Acid Content and Composition in Microalgae

Published on: October 1, 2013

Area of Science:

* Biotechnology
* Microalgal cultivation
* Cell sorting technologies

Background:

* Efficiently identifying and isolating high-lipid microalgal strains is crucial for biofuel and bioproduct applications.
* Traditional methods for lipid detection in microalgae can be time-consuming and may affect cell viability.
* Fluorescence activated cell sorting (FACS) offers a high-throughput approach for cell analysis and isolation.

Purpose of the Study:

* To establish an optimized and efficient staining protocol for fluorescence activated cell sorting (FACS) of Chlorococcum littorale.
* To identify a fluorescent dye suitable for detecting high-lipid content in C. littorale while ensuring cellular viability.
* To develop a method for rapid screening and sorting of lipid-rich C. littorale cells for enhanced inoculum production.

Main Methods:

* Evaluation of BODIPY505/515 (BP) and Nile red as fluorescent stains for lipid detection in C. littorale via FACS.
* Optimization of BP staining concentrations (0.4 μg ml⁻¹) and solvent (0.1% DMSO or 0.35% ethanol).
* Assessment of cellular viability post-staining and sorting using both ethanol and DMSO as solvents.

Main Results:

* BODIPY505/515 (BP) demonstrated superior suitability for FACS compared to Nile red for lipid detection in C. littorale.
* Optimal staining conditions were determined as 0.4 μg ml⁻¹ BP with either 0.1% DMSO or 0.35% ethanol.
* Both DMSO and ethanol effectively maintained cellular viability after staining and sorting procedures.

Conclusions:

* An efficient and viable staining method for FACS-based lipid screening in C. littorale has been successfully established.
* The developed method enables rapid identification and isolation of high-lipid C. littorale cells.
* This technique facilitates the production of improved inoculum with enhanced cellular lipid content for biotechnological applications.