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Preparation of Samples for Electron Microscopy01:20

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To be visualized by an electron microscope, either transmission or scanning, biological samples need to be fixed (stabilized) so the electron beam does not destroy them and dried thoroughly (desiccated/dehydrated) so the vacuum does not affect them. Fixation needs to be done as quickly as possible because the sample properties will start changing as soon as it is removed from its natural environment. For example, in a tissue sample, the oxygen levels begin decreasing, causing an altered...
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RP implementation: specimen management.

Patricia Graybill-D'Ercole

    AORN Journal
    |December 3, 2014
    PubMed
    Summary
    This summary is machine-generated.

    Effective specimen management in perioperative settings is crucial for accurate patient diagnosis. AORN's recommended practices guide nurses and multidisciplinary teams through the entire specimen lifecycle, minimizing errors.

    Keywords:
    explantsurgical pathologysurgical specimentissue preservation

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    Area of Science:

    • Perioperative Nursing
    • Surgical Pathology
    • Healthcare Quality Improvement

    Background:

    • Accurate patient diagnosis and intervention depend on effective perioperative specimen management.
    • The Association of periOperative Registered Nurses (AORN) provides essential guidance for this process.

    Purpose of the Study:

    • To outline AORN's "Recommended practices for specimen management" for perioperative settings.
    • To emphasize the importance of a multidisciplinary approach and error awareness in specimen handling.

    Main Methods:

    • Review and synthesis of AORN's "Recommended practices for specimen management" document.
    • Identification of key components within the specimen management process, from collection to documentation.

    Main Results:

    • The recommended practices cover a comprehensive specimen management process: needs assessment, site identification, collection, handling, transfer, containment, labeling, preservation, transport, disposition, and documentation.
    • Specific specimen types addressed include breast cancer, amputated limbs, forensic, radioactive, and explanted devices.
    • Effective multidisciplinary communication is highlighted as critical for minimizing errors.

    Conclusions:

    • Implementing AORN's recommended practices ensures standardized and accurate specimen management.
    • These guidelines are applicable in both hospital and ambulatory surgical settings, enhancing patient safety and diagnostic accuracy.