Related Experiment Video
Updated: Apr 20, 2026
12:37
Phase Diagram Characterization Using Magnetic Beads as Liquid Carriers
Published on: September 4, 2015
13.1K
Determination of aqueous two phase system binodal curves using a microfluidic device
D F C Silva1, A M Azevedo2, P Fernandes2
1INESC Microsistemas e Nanotecnologias and IN-Institute of Nanoscience and Nanotechnology, Lisbon, Portugal; IBB - Institute for Biotechnology and Bioengineering, Centre for Biological and Chemical Engineering, Instituto Superior Técnico, Universidade de Lisboa, Lisbon, Portugal.
Journal of Chromatography. A
|December 3, 2014
Summary
This study introduces a microfluidic device for rapidly determining aqueous two-phase system (ATPS) binodal curves, overcoming limitations of traditional methods for biomolecule separation.
Area of Science:
- Biochemistry
- Chemical Engineering
- Separation Science
Background:
- Aqueous two-phase systems (ATPS) are promising for biomolecule separation but hindered by poor theoretical understanding and slow parameter determination.
- Traditional methods for determining ATPS binodal curves are empirical and time-consuming.
Purpose of the Study:
- To develop a novel, rapid microfluidic technique for determining ATPS binodal curves.
- To improve the understanding and application of ATPS for biomolecule separation.
Main Methods:
- A microfluidic device was used with three inlets for ATPS precursors and water.
- Varying flow rates generated a range of concentrations for rapid testing with limited volumes.
- Optical microscopy identified three states: interface, no interface, and unstable interface.
Main Results:
- Binodal curves were calculated using points of unstable interfaces.
- The microfluidic method rapidly determined binodal curves for polyethylene glycol (PEG)/salt and PEG/dextran systems.
- Results were comparable to the standard turbidometric titration method.
Conclusions:
- The microfluidic technique offers a faster and more efficient alternative for determining ATPS binodal curves.
- This method facilitates the optimization of ATPS for biomolecule separation.
- Improved understanding and application of ATPS are expected.
