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Updated: Aug 15, 2026

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Genome-wide Gene Deletions in Streptococcus sanguinis by High Throughput PCR
Published on: November 23, 2012
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Colony polymerase chain reaction of heme-accumulating bacteria
Bikiran Pardesi1, Geoffrey R Tompkins1
1Sir John Walsh Research Institute, University of Otago, 310 Great King St, Dunedin 9054, New Zealand.
Anaerobe
|December 3, 2014
Abstract:
Colony PCR of anaerobic black-pigmenting Bacteroidetes species Porphyromonas gingivalis and Prevotella intermedia was modified by addition of bovine serum albumin to reverse the inhibitory action of accumulated heme.
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DNA replication has three main steps: initiation, elongation, and termination. Replication in prokaryotes begins when initiator proteins bind to the single origin of replication (ori) on the cell's circular chromosome. Replication then proceeds around the entire circle of the chromosome in each direction from the two replication forks, resulting in two DNA molecules.
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The DNA replication, transcription, and translation processes are intricately coupled in bacteria, allowing efficient gene expression and rapid protein synthesis. While this physical and functional coordination is advantageous, it introduces challenges that bacteria overcome through specific regulatory mechanisms.Coupling of Replication, Transcription, and TranslationThe coupling of replication, transcription, and translation is a hallmark of bacterial gene expression. As the replisome unwinds...

