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An evolutionarily conserved non-coding element in casein locus acts as transcriptional repressor.

Suneesh Kaimala1, Satish Kumar1

  • 1Centre for Cellular and Molecular Biology, Council of Scientific and Industrial Research (CSIR), Hyderabad, India.

Gene
|December 3, 2014
PubMed
Summary

Researchers discovered a novel regulatory element in the mammalian casein locus. This evolutionarily conserved region (ECR) acts as a repressor, potentially controlling Odam gene expression in the mammary gland.

Keywords:
ECRMammary glandOdam

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Area of Science:

  • Genomics
  • Molecular Biology
  • Gene Regulation

Background:

  • The mammalian casein locus contains non-coding DNA with largely unknown functions.
  • These regions are hypothesized to contain elements regulating spatio-temporal gene expression.
  • Few regulatory elements within the casein locus have been identified to date.

Purpose of the Study:

  • To identify and characterize novel regulatory elements within the mammalian casein locus.
  • To investigate the function of a newly identified evolutionarily conserved region (ECR).

Main Methods:

  • Comparative genomic DNA sequence analysis across different mammalian species.
  • In-silico prediction of regulatory element function.
  • Functional analysis using various cell lines.

Main Results:

  • A 147bp evolutionarily conserved region (ECR) was identified upstream of the Odam gene in the casein locus.
  • The ECR was found to be a unidirectional repressor element.
  • The ECR is conserved across all examined mammalian species and located near the Odam gene.

Conclusions:

  • A novel unidirectional repressor element (ECR) has been identified in the casein locus.
  • This ECR may play a role in repressing Odam gene expression in the mammary gland during lactation.