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Sleeping Beauty transposon system for genetic etiological research and gene therapy of cancers

Xiaomei Hou1, Yan Du, Yang Deng

  • 1a Department of Epidemiology ; Second Military Medical University ; Shanghai , China.

Cancer Biology & Therapy
|December 3, 2014
PubMed

Insights

The Sleeping Beauty transposon system aids in understanding cancer progression and developing gene therapies. This powerful tool helps validate gene mutations and molecules involved in carcinogenesis and metastasis.

Area of Science:

  • Genetics
  • Molecular Biology
  • Oncology

Background:

  • Carcinogenesis is linked to somatic mutations in critical genes.
  • Functional networks of mutations and molecules influence cancer progression.
  • Animal models are essential for validating cancer-related gene functions.

Purpose of the Study:

  • To review advances in the Sleeping Beauty transposon system for cancer research.
  • To highlight its role in understanding cancer mechanisms and gene therapy.

Main Methods:

  • Utilizing the Sleeping Beauty transposon system, which involves a transposon carrying genes of interest and a transposase.
  • Employing this system in animal models to create gain-of-function and loss-of-function mutations.
  • Summarizing current research employing this system.

Main Results:

  • The Sleeping Beauty transposon system facilitates the investigation of etiological mechanisms in cancer.
  • It is instrumental in developing and testing gene therapy strategies for various cancers.
  • Advances in this system enhance the understanding of molecular drivers of carcinogenesis.

Conclusions:

  • The Sleeping Beauty transposon system is a valuable tool for studying cancer molecular mechanisms.
  • It aids in validating the roles of specific genes and molecules in cancer development and progression.
  • Insights gained can lead to novel prophylactic and therapeutic strategies for malignant diseases.
Keywords:
7, 12-dimethylbenzanthracene/12-O-tetradecanoylphorbol-13-acetateAlb-Cre, Albumin promoter-CreCAG promoter, CMV enhancer/chicken β-actin promoterCAR, chimeric antigen receptorCIS, common insertion siteCMV, chimeric cytomegalovirusCRC, colorectal cancerCre, cyclization recombination enzymeDDE, Asp, Asp, GluDMBA/TPADR, direct orientationFah, fumarylacetoacetate hydrolase geneGWAS, gnome wide analysis studyHBV, Hepatitis B VirusHBx, HBV X proteinHCC, hepatocellular carcinomaIRs, inverted repeat sequencesLsL, loxP-stop-loxPMPNSTs, malignant peripheral nerve sheath tumorMSCV, murine stem cell virusPAI, Pro, Ala, IlePBMCs, peripheral blood mononuclear cellsRED, Arg, Glu, AspRosaSBaseLsL, Cre-inducible SBase alleleRtl1, Retrotransposon-like 1SB, Sleeping BeautySBase, Sleeping Beauty transposaseSleeping Beauty transposon systemStatinAE, angiostatin-endostatin fusion geneTrp53, transformation related protein 53animal modeldrivergene functiongene therapymalignant diseasessgRNA, single guide RNAshp53, short hairpin RNA against the Trp53 genesomatic mutation

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