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Generation of Human Monocyte-derived Dendritic Cells from Whole Blood
Published on: December 24, 2016
Menstrual blood-derived stromal stem cells inhibit optimal generation and maturation of human monocyte-derived
Mahmood Bozorgmehr1, Seyed Mohammad Moazzeni2, Mojdeh Salehnia3
1Immunology Department, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran; Nanobiotechnology Research Center, Avicenna Research Institute, ACECR, Tehran, Iran.
Insights
Menstrual blood stromal stem cells (MenSCs) inhibit the generation and maturation of dendritic cells (DCs). This immune modulation may involve IL-6 and IL-10, suggesting MenSCs could impact immune responses.
Area of Science:
- Stem cell biology
- Immunology
- Cell biology
Background:
- Menstrual blood stromal stem cells (MenSCs) show clinical potential but their immune interactions are poorly understood.
- Dendritic cells (DCs) are crucial for regulating immune responses.
Purpose of the Study:
- To investigate the effect of MenSCs on the generation and maturation of human blood monocyte-derived dendritic cells (DCs).
Main Methods:
- MenSCs were isolated from menstrual blood.
- Monocytes were differentiated into immature (iDC) and mature (mDC) DCs with or without MenSCs.
- Cell markers, costimulatory molecules, IL-6, and IL-10 were analyzed.
Main Results:
- MenSCs inhibited monocyte differentiation into iDCs.
- IL-6 and IL-10 levels were elevated in MenSC-monocyte cocultures.
- MenSCs hindered the generation of fully mature DCs when added early in the differentiation process.
Conclusions:
- MenSCs exert an inhibitory impact on DC generation and maturation.
- Elevated IL-6 and IL-10 may partly mediate this effect.
- Further research into MenSCs' immunomodulatory potential is warranted.
Introduction:
Menstrual blood stromal stem Cells (MenSCs) have shown promising potential for future clinical settings. Nonetheless, data regarding their interaction with immune cells is still scarce. Here, we investigated whether MenSCs could affect the generation and/or maturation of human blood monocyte-derived dendritic cells (DCs).
Materials And Methods:
MenSCs were isolated from menstrual blood of normal women through culture of adherent mononuclear cells. Magnetically-isolated peripheral blood monocytes were differentiated toward immature DCs (iDC) and mature DCs (mDCs) in the presence or absence of MenSCs. Monocyte-derived cells were assessed for the percentage of monocyte-, iDC-, and mDC-specific markers as well as the expression of costimulatory molecules. IL-6 and IL-10 levels were also determined in supernatants of MenSC-monocytes cocultures.
Results:
Optimal phenotypic differentiation of monocytes into iDCs was inhibited upon coculture with MenSCs. Moreover, higher levels of IL-6 and IL-10 were detected in these settings. Even though addition of MenSCs to iDC cultures could not prevent iDC maturation, coculture of MenSCs with monocytes from the beginning of differentiation process could effectively hinder generation of fully mature DCs.
Conclusion:
This is the first study to address the inhibitory impact of MenSCs on generation and maturation of DCs. IL-6 and IL-10 could be partly held responsible for this effect. Given the central roles of DCs in regulation of immune responses, these results highlight the importance of further research on the potential modulatory impacts of MenSCs, as rather easily accessible and expandable stem cells, on the immune system-related cells.
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