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Related Experiment Videos

Moderate Fuchs' endothelial dystrophy ATPase pump site density.

M D McCartney1, T O Wood, B J McLaughlin

  • 1Department of Ophthalmology and Visual Sciences, University of Louisville School of Medicine, Kentucky Lions Eye Research Institute, Louisville 40202.

Investigative Ophthalmology & Visual Science
|July 1, 1989
PubMed
Summary

Fuchs

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Area of Science:

  • Ophthalmology
  • Cell Biology
  • Biochemistry

Background:

  • Fuchs' endothelial dystrophy impacts corneal endothelial cells.
  • Na+, K+-ATPase pump site density varies in disease stages.

Purpose of the Study:

  • Quantify Na+, K+-ATPase pump site density in intermediate stages of Fuchs' endothelial dystrophy.
  • Characterize the progression of pump site density changes.

Main Methods:

  • Utilized autoradiography and immunohistochemistry on eye bank corneas.
  • Examined corneas with guttata but no edema.

Main Results:

  • Pump site density was elevated compared to end-stage disease but lower than functional or early-stage tissue.
  • Immunohistochemistry confirmed increased Na+, K+-ATPase antibody localization versus end-stage disease, but reduced versus functional tissue.

Conclusions:

  • Endothelial cell Na+, K+-ATPase pump site density in Fuchs' dystrophy gradually declines.
  • The deterioration is not a sudden sharp decrease after an initial increase.

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