Intracellular synthesis of silver nanoparticle by actinobacteria and its antimicrobial activity
S V Otari1, R M Patil1, S J Ghosh1
1Center for Interdisciplinary Research, D.Y. Patil University, Kolhapur 416 006, Maharashtra State, India.
Abstract:
Intracellular synthesis of silver nanoparticles (AgNPs) using Rhodococcus spp. is demonstrated. The synthesized nanoparticles were characterized by UV-Vis spectroscopy, X-ray diffraction, energy dispersive spectroscopy, Fourier trans-form infrared spectroscopy, and transmission electron microscopy. Transmission electron microscopy study of microorganisms' revealed synthesis of nanoparticle was occurring inside the cell, in the cytoplasm. AgNPs ranged from 5 to 50 nm. Formed nanoparticles were stable in the colloidal solution due to presence of proteins on the surface. AgNPs showed excellent bactericidal and bacteriostatic activity against pathogenic microorganisms.
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