Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

RNA Polymerase II Accessory Proteins

RNA Polymerase II Accessory Proteins

Proteins that regulate transcription can do so either via direct contact with RNA Polymerase or through indirect interactions facilitated by adaptors, mediators, histone-modifying proteins, and nucleosome remodelers. Direct interactions to activate transcription is seen in bacteria as well as in some eukaryotic genes. In these cases, upstream activation sequences are adjacent to the promoters, and the activator proteins interact directly with the transcriptional machinery. For example, in...

RNA Polymerase II Accessory Proteins

RNA Polymerase II Accessory Proteins

Chromatin Structure Regulates pre-mRNA Processing

Chromatin Structure Regulates pre-mRNA Processing

In eukaryotic cells, nascent mRNA transcripts need to undergo many post-transcriptional modifications to reach the cell cytoplasm and translate into functional proteins. For a long time, transcription and pre-mRNA processing were considered two independent events that occur sequentially in the cell. However, it has now been well established that transcription and pre-mRNA processing are two simultaneous processes that are precisely regulated inside the cell.
The chromatin structure, especially...

Chromatin Position Affects Gene Expression

Chromatin Position Affects Gene Expression

Chromatin is the massive complex of DNA and proteins packaged inside the nucleus. The complexity of chromatin folding and how it is packaged inside the nucleus greatly influences access to genetic information. Generally, the nucleus' periphery is considered transcriptionally repressive, while the cell's interior is considered a transcriptionally active area.
Topologically Associated Domains (TADs)
The 3-dimensional positioning of chromatin in the nucleus influences the...

Co-activators and Co-repressors

Co-activators and Co-repressors

Gene transcription is regulated by the synergistic action of several proteins that form a complex at a gene regulatory site. This is observed in eukaryotes, where the regulation of gene expression is a complex process. Regulatory proteins in eukaryotes can broadly be classified into two types – regulators that bind directly to specific DNA sequences and co-regulators that associate with regulatory proteins but cannot directly bind to the DNA. These co-regulators are further divided into...

Co-activators and Co-repressors

Co-activators and Co-repressors

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Cholesterol-driven sequestration of RETREG1/FAM134B regulates ERphagy and STING1 innate immunity.

Autophagy·2026

Same author

MYCN drives pediatric glioma transformation from neural progenitors and creates distinct therapeutic vulnerabilities.

Cell reports·2026

Same author

Identification of a conserved receptor for degrading ribosomes through autophagy.

Autophagy·2026

Same author

Dominant clones leverage developmental epigenomic states to drive ependymoma.

Nature·2026

Same author

Unravelling phenotypic variations and establishing a core collection in mungbean for accelerating the crop improvement programs.

Frontiers in plant science·2026

Same author

Relationship between the distribution of LEDGF along genes and positions of HIV-1 DNA integration.

mBio·2026

Search research articles

Related Experiment Video

Updated: Apr 20, 2026

HOX Loci Focused CRISPR/sgRNA Library Screening Identifying Critical CTCF Boundaries

HOX Loci Focused CRISPR/sgRNA Library Screening Identifying Critical CTCF Boundaries

Published on: March 31, 2019

The RSC complex localizes to coding sequences to regulate Pol II and histone occupancy.

Marla M Spain¹, Suraiya A Ansari², Rakesh Pathak¹

¹Department of Biological Sciences, Oakland University, Rochester, MI 48309, USA.

|December 3, 2014

Summary

The RSC ( a chromatin remodeler) is recruited to actively transcribed genes, regulating transcription elongation and maintaining chromatin structure. Its depletion reduces Pol II and histone H3 occupancy, impacting gene expression.

More Related Videos

Chromatin Isolation by RNA Purification ChIRP

Chromatin Isolation by RNA Purification ChIRP

Published on: March 25, 2012

Repressing Gene Transcription by Redirecting Cellular Machinery with Chemical Epigenetic Modifiers

Repressing Gene Transcription by Redirecting Cellular Machinery with Chemical Epigenetic Modifiers

Published on: September 20, 2018

Related Experiment Videos

Last Updated: Apr 20, 2026

HOX Loci Focused CRISPR/sgRNA Library Screening Identifying Critical CTCF Boundaries

HOX Loci Focused CRISPR/sgRNA Library Screening Identifying Critical CTCF Boundaries

Published on: March 31, 2019

Chromatin Isolation by RNA Purification ChIRP

Chromatin Isolation by RNA Purification ChIRP

Published on: March 25, 2012

Repressing Gene Transcription by Redirecting Cellular Machinery with Chemical Epigenetic Modifiers

Repressing Gene Transcription by Redirecting Cellular Machinery with Chemical Epigenetic Modifiers

Published on: September 20, 2018

Area of Science:

Molecular Biology
Gene Regulation
Chromatin Dynamics

Background:

ATP-dependent chromatin remodelers are crucial for regulating gene transcription.
The RSC complex is an essential chromatin remodeler with a poorly understood role in transcription.

Purpose of the Study:

To investigate the genome-wide localization and function of the RSC complex during transcription.
To determine the role of RSC in transcription elongation and chromatin maintenance over actively transcribed genes.

Main Methods:

Genome-wide chromatin immunoprecipitation (ChIP) to map RSC occupancy.
RNA sequencing and ChIP to assess Pol II and histone H3 occupancy upon RSC depletion.
Genetic analysis of RSC recruitment in conjunction with SAGA, NuA4, Bur1, and Ctk1 activities.

Main Results:

RSC is recruited to the open reading frames (ORFs) of actively transcribed genes genome-wide.
Depletion of the RSC catalytic subunit Sth1 reduces Pol II and histone H3 occupancy in ORFs, particularly for weakly transcribed genes.
RSC recruitment to ORFs depends on SAGA, NuA4 HAT complexes, and Pol II CTD Ser2 kinases Bur1 and Ctk1.

Conclusions:

ORF-associated RSC plays a critical role in regulating transcription elongation by RNA Polymerase II.
RSC is essential for maintaining proper chromatin structure over transcribed regions.
RSC function is integrated with other transcriptional regulatory complexes and kinases.