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Updated: Apr 20, 2026
Gene Transfection toward Spheroid Cells on Micropatterned Culture Plates for Genetically-modified Cell Transplantation
Published on: July 31, 2015
Effective modulation of transforming growth factor-β1 expression through engineered microRNA-based plasmid-loaded
Youlang Zhou1, Changlai Zhu2, Ya Fang Wu1
1Hand Surgery Research Center, Department of Hand Surgery, Affiliated Hospital of Nantong University, Nantong, Peoples Republic of China.
Background Aims:
Sustained gene delivery could be particularly necessary in modulating gene expression in healing intrasynovial tendons and may be a promising approach for preventing adhesions.
Methods:
Polylactic-co-glycolic acid nanospheres were prepared and were then incorporated with plasmids expressing enhanced green fluorescence protein and miRNA for inhibiting the transforming growth factor-β1 gene expression.
Results:
We demonstrated that cultured tenocytes could be effectively transfected by means of nanosphere/plasmid. The expression of transforming growth factor-β1 is significantly downregulated in healing chicken flexor tendon treated with nanosphere/plasmid. Histology analysis did not demonstrate any significant inflammation or necrosis in tendons injected with nanosphere/plasmid after surgery.
Conclusions:
Nanosphere/plasmid may be a promising non-viral vector for gene therapy of the injured flexor tendon.
