In situ hybridization for quantitative assay of infectious hepatitis A virus

¹Division of Molecular Virology, Baylor College of Medicine, Houston, Texas 77030.

|May 1, 1989

Abstract:

A method of in situ hybridization using single-stranded RNA probes of opposite polarity for quantitative enumeration of hepatitis A virus (HAV) in infected cells has been developed. Kinetic experiments showed that foci of infected cells appeared as early as day 2 postinfection. The absence of foci in cells examined immediately after virus adsorption indicated that foci detected subsequently were related to viral replication. Foci were detected by hybridization with RNA probes complementary to HAV genomic RNA but not with RNA probes identical to HAV genomic RNA. The number of foci observed was linearly related to the HAV dose inoculated. Focus formation was reduced when a virus inoculum was pretreated with guinea pig anti-HAV hyperimmune serum but not when it was pretreated with preimmune serum. The high resolution of hybridization signals and relative rapidity of the test indicated that this technique will be useful for measuring serum neutralizing antibodies and for quantitative assay of infectious HAV.

Related Experiment Videos

In situ hybridization for quantitative assay of infectious hepatitis A virus

Related Concept Videos

[Clinical profiles of community-acquired Pseudomonas aeruginosa infections in children].

[Application of electrical impedance tomography in pulmonary rehabilitation].

[A survey on clinical practice of tracheostomy decannulation in medical institutions at all levels across the country].

A Method for Recovery of Viruses from Oysters and Hard and Soft Shell Clams.

Noroviruses as a Cause of Diarrhea in Immunocompromised Pediatric Hematopoietic Stem Cell and Solid Organ Transplant Recipients.

Electron cryomicroscopy and computer image processing techniques : use in structure-function studies of rotavirus.

Breath aerosol PCR for detection of lower respiratory tract infections: evaluation of a non-invasive face mask collector in pneumonia patients.

When tests don't fit the rules: regulatory challenges for agnostic metagenomic next-generation sequencing in infectious diseases diagnostics.

Benchmarking four commercial antimicrobial susceptibility testing systems and disk diffusion to reference broth microdilution in the assessment of gram-negative bacterial isolates.

Update on novel, validly published, and included bacterial taxa derived from human clinical specimens and taxonomic revisions published in 2025.

Development and evaluation of an ARTIC-based amplicon sequencing assay for whole-genome characterization of respiratory syncytial virus.

A novel biomarker signature based on circulating transfer RNA (tRNA) can detect and differentiate HIV-2 infection from HIV-1.

Commensal bacteria inhibit viral infections via a tryptophan metabolite.

Molecular identification of NF-κB subunit RelA in Manila clam (Ruditapes philippinarum) and its regulatory role in inflammatory response during Vibrio parahaemolyticus infection.

Metagenomic next-generation sequencing contributes to the diagnosis of Gardnerella vaginalis bacteremia associated with a rare extragenital infection and diagnostic challenges in a 13-year-old female patient: A case report.

ARID5A RNA-binding coordinates microglial defense and ferroptosis in iPSC-derived models.

Quantifying Viral Lysis in Microalgae Using Cell-Free rRNA.

Single-Dye, Transfection-Free FLIM Multiplexing via Bioorthogonal Chemistry.

Related Experiment Videos

In situ hybridization for quantitative assay of infectious hepatitis A virus

Abstract:

Related Concept Videos

Related Articles