Related Experiment Video
Updated: Apr 20, 2026
Evaluation of a Reliable Biomarker in a Cecal Ligation and Puncture-Induced Mouse Model of Sepsis
Published on: December 9, 2022
Serum citrulline as a diagnostic marker of sepsis-induced intestinal dysfunction
Li-Juan Shen1, Yun-Yan Guan1, Xi-Ping Wu1
1Department of ICU, Wuxi Hospital of traditional Chinese Medicine, Nanjing University of Chinese Medicine Affiliated Wuxi Hospital, No. 8, South West Road, Wuxi 214071, Jiangsu, China.
Objective:
To investigate the use of citrulline as an indicator for diagnosing septic acute intestinal dysfunction (SAID) in a rat model.
Methods:
SD rats were divided into three groups: a normal group (A), a model group (B), and a glutamine group (C). Group B was divided into a 36-h group (B1) and a 72-h feeding group (B2). The concentrations of serum citrulline, intestinal fatty acid-binding protein (I-FABP) and intestinal glutamine and histopathological changes were measured.
Results:
The lengths of the villus and thicknesses of the mucosal layer in groups B1, B2 and C were significantly different from those in group A. Citrulline concentrations in groups B1, B2 and C were lower than in group A; the serum concentrations in group C were significantly greater than in groups B1 and B2. The I-FABP levels of groups B1, B2 and C were higher than group A; I-FABP levels of groups B1 and B2 were higher than group C. Intestinal glutamine levels of groups B1 and B2 were lower than groups A and C. The serum citrulline of group C was negatively correlated with I-FABP and Chiu's score.
Conclusions:
Serum citrulline could be used as the diagnostic indicator of SAID.
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