Related Experiment Video
Updated: Apr 20, 2026

High-throughput Saccharification Assay for Lignocellulosic Materials
Published on: July 3, 2011
Influence of organic loading rate and solid retention time on polyhydroxybutyrate production from hybrid poplar
Jing Dai1, Karol Gliniewicz2, Matthew L Settles2
1Department of Forest, Rangeland and Fire Sciences, University of Idaho, Moscow, ID 83844-1132, United States.
Abstract:
The aim of this study was to investigate the potential of using wood hydrolysates (enzymatically hydrolyzed from hybrid poplar) as substrate to produce polyhydroxybutyrate (PHB) using mixed microbial cultures. The optimal operational conditions for fed-batch bioreactors were 4d solid retention time with an organic loading rate of 2.5g/Ld. The maximum PHB accumulated was 27% of cell dry weight with a yield of 0.32g/g (g PHB produced per g sugars consumed). Microbial community analysis was done at the genus level by 16S rRNA sequencing on an Illumina system and community evolution was observed among different samples and initial seed. Actinobacteria, Alpha- and Beta-proteobacteria were found to be the dominant groups in all the bioreactors. Several PHB-storing microorganisms were characterized belonging to Alpha- and Beta-proteobacteria.
More Related Videos
07:24Production of Chemicals by Klebsiella pneumoniae Using Bamboo Hydrolysate as Feedstock
Published on: June 29, 2017
16:33Methods for Facilitating Microbial Growth on Pulp Mill Waste Streams and Characterization of the Biodegradation Potential of Cultured Microbes
Published on: December 12, 2013
Related Concept Videos
Bioplastics
Production of Organic Acids
Microbial Bioremediation of Plastics