Enzymatic hydrolysis of steam-exploded sugarcane bagasse using high total solids and low enzyme loadings
Luiz Pereira Ramos1, Larissa da Silva2, Annielly Comelli Ballem2
1Research Center in Applied Chemistry (CEPESQ), Department of Chemistry, Federal University of Paraná, Curitiba, PR, Brazil; INCT in Energy & Environment, Department of Chemistry, Federal University of Paraná, Brazil.
Abstract:
Hydrolysis of phosphoric acid-impregnated steam-treated sugarcane bagasse was pre-optimized using a face-centered central composite design in which the process variables were the substrate total solids (TS, %), agitation intensity (AI, rpm) and enzyme loading (EL, gg(-1)). Pretreatment was carried out at 180°C for 10min using cane bagasse with 50wt% moisture content containing 9.5mg of H3PO4 per gram of dry biomass. Hydrolyses were performed for 96h at 50°C using Cellic CTec2® and water-washed steam-treated substrates. The highest amount of fermentable sugars was obtained with 20wt% TS, producing 76.8gL(-1) of glucose equivalents, which corresponded to a total glucan conversion of 69.2wt% and to a theoretical net increase of 39% in ethanol production from the same sugarcane tonnage without considering the use of leaves, tops and the additional yields from C5 sugars.
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