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A Microfluidic Chip for the Versatile Chemical Analysis of Single Cells
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Jetting microfluidics with size-sorting capability for single-cell protease detection.

Tengyang Jing1, Ramesh Ramji2, Majid Ebrahimi Warkiani3

  • 1Department of Biomedical Engineering, National University of Singapore, Singapore 117575, Singapore; Singapore-MIT Alliance for Research and Technology (SMART) Centre, Singapore 1385602, Singapore.

Biosensors & Bioelectronics
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Summary
This summary is machine-generated.

This study introduces a microfluidic device for analyzing matrix metalloproteinase (MMP) secretion from individual cancer cells. The platform effectively isolates single cells to reveal heterogeneity in MMP activity and drug responses.

Keywords:
Cell encapsulationDLD droplet sortingJetting microfluidicsProtease biosensorSingle-cell analysis

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Area of Science:

  • Biotechnology
  • Cancer Research
  • Microfluidics

Background:

  • Activated matrix metalloproteinases (MMPs) secreted by cancer cells degrade the extracellular matrix (ECM), promoting tumor growth and metastasis.
  • Assessing MMP activity at the single-cell level is crucial for understanding cancer cell heterogeneity and disease progression.

Purpose of the Study:

  • To develop a microfluidic platform for encapsulating individual cancer cells in picoliter droplets for single-cell analysis.
  • To measure kinetic profiles of MMP secretion and drug responses in heterogeneous cancer cell populations.

Main Methods:

  • A microfluidic system combining droplet jetting and deterministic lateral displacement (DLD) size-sorting was utilized.
  • Cell-triggered Rayleigh-Plateau instability generated droplets for efficient single-cell encapsulation.
  • DLD channels separated single-cell droplets, achieving ~78% enrichment regardless of input cell density.

Main Results:

  • The platform successfully encapsulated individual cancer cells in picoliter droplets.
  • Single-cell analysis revealed heterogeneous MMP secretion activities among cancer cells.
  • The inhibitory response to doxycycline was characterized at the single-cell level.

Conclusions:

  • The developed microfluidic platform enables effective single-cell encapsulation and analysis of MMP secretion.
  • This technology provides insights into cancer cell heterogeneity and drug responses.
  • It offers a valuable tool for advancing cancer research and personalized medicine.