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Methanol Independent Expression by Pichia Pastoris Employing De-repression Technologies
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Improved propionic acid production from glycerol: combining cyclic batch- and sequential batch fermentations with

Tarek Dishisha1, Mohammad H A Ibrahim1, Victor Hugo Cavero1

  • 1Department of Biotechnology, Center for Chemistry and Chemical Engineering, Lund University, P.O. Box 124, SE-221 00 Lund, Sweden.

Bioresource Technology
|December 3, 2014
PubMed
Summary

Optimizing yeast extract concentration in Propionibacterium acidipropionici fermentation significantly boosts propionic acid production. High cell density strategies, like sequential batch fermentation with cell recycling, achieve high yields and productivity from glycerol.

Keywords:
Carbon:nitrogen ratioCyclic batch fermentationPropionibacterium acidipropioniciPropionic acidSequential batch fermentation

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Area of Science:

  • Biotechnology
  • Industrial Microbiology
  • Metabolic Engineering

Background:

  • Glycerol is a byproduct of biodiesel production, presenting an opportunity for valorization.
  • Propionic acid has diverse industrial applications, including food preservation and pharmaceuticals.
  • Efficient microbial production of propionic acid from glycerol is crucial for sustainable chemical manufacturing.

Purpose of the Study:

  • To investigate the impact of carbon and nitrogen source concentrations on propionic acid production by Propionibacterium acidipropionici.
  • To evaluate different high cell density fermentation strategies for enhanced process kinetics and efficiency.
  • To determine the optimal glycerol to yeast extract ratio for maximizing propionic acid yield and productivity.

Main Methods:

  • Utilized Propionibacterium acidipropionici for glycerol fermentation.
  • Employed three-way ANOVA and batch cultivations to analyze the effects of varying C/N ratios and yeast extract concentrations.
  • Implemented cyclic batch fermentation and sequential batch fermentation with cell recycling for high cell density cultivation.

Main Results:

  • Yeast extract concentration significantly influenced propionic acid production rate.
  • An optimal glycerol to yeast extract ratio (3:1, w/w) was identified for complete glycerol consumption and sustained volumetric productivity.
  • Cyclic batch fermentation achieved a propionate yield of 93 mol% and a productivity of 0.53 g/L/h.
  • Sequential batch fermentation with cell recycling exceeded 1 g/L/h productivity with initial glycerol up to 120 g/L, reaching a maximum of 1.63 g/L/h from 90 g/L glycerol.

Conclusions:

  • Yeast extract concentration is a critical factor for optimizing propionic acid production from glycerol.
  • High cell density fermentation strategies, particularly sequential batch fermentation with cell recycling, are effective for achieving high volumetric productivity.
  • This study provides a foundation for developing efficient and sustainable industrial processes for propionic acid biosynthesis from glycerol.