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Detection of Toxin Translocation into the Host Cytosol by Surface Plasmon Resonance
Published on: January 3, 2012
An aptamer based surface plasmon resonance biosensor for the detection of ochratoxin A in wine and peanut oil
Zhiling Zhu1, Mengxue Feng1, Limin Zuo2
1Beijing Key Laboratory of Bioprocess, College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029 China; Institute of Medicinal Biotechnology, Chinese Academy of Medical Science and Peking Union Medical College, Beijing 100050 China.
Abstract:
Ochratoxin A (OTA), as a kind of chlorophenolic mycotoxin, exist widely in plant origin food and is harmful to human. Herein, a surface plasmon resonance (SPR) biosensor using an anti-OTA aptamer immobilized sensor chip was developed to measure ochratoxin A (OTA) quantificationally through a straightforward direct binding assay. The streptavidin protein as a crosslinker was immobilized onto the surface of a sensor chip and the biotin-aptamer was captured through streptavidin-biotin interaction. The biosensor exhibited a detection range from 0.094 to 100ng/mL (linear range from 0.094 to 10ng/mL) of OTA with a lower detection limit of 0.005ng/mL. Detection of OTA in wine and peanut oil was further performed in the SPR biosensor using simple liquid-liquid extraction for sample pretreatments. Recoveries of ochratoxin A from spiked samples ranged from 86.9% to 116.5% and coefficients of variation (CVs) ranged from 0.2% to 6.9%. The developed methods in our studies showed good analytical performances with limits of detection much lower than the maximum residue limit, as well as a good reproducibility and stability.
