Interactive effects of mercury and arsenic on their uptake, speciation and toxicity in rice seedling

Rice can take up and translocate more As and Hg than other cereal crops. A hydroponic experiment was conducted to investigate their interactive effects on their uptake and toxicity in rice seedling after exposing to As(III) (0.1, 0.5 or 2.5 mg L−1) and Hg (0.05, 0.25 or 1.25 mg L−1) for 14 d. Rice was much more effective in taking up Hg than As and sequestered both in the roots. As and Hg reached 339 and 433 mg kg−1 in the roots, and 48.5 and 16.1 mg kg−1 in the shoots at As2.5 + Hg1.25. Though Hg inhibited As uptake and translocation, it enhanced As(III) toxicity to rice seedling. However, As inhibited Hg uptake at Hg0.05, but the opposite was observed at Hg0.25 and Hg1.25. Arsenite (54–100%) and inorganic Hg (100%) were the predominant form in the plant based on speciation analysis via HPLC–ICP–MS. Malondialdehyde in the roots and shoots increased with increasing As and Hg concentrations, with the highest being 54 μmol g−1 at As0.5 + Hg1.25 in the roots. Root cell structural damage and organelles number reduction with increasing As and Hg concentration were observed based on TEM. As and Hg transformation and toxicity can help to understand the metabolic mechanisms of As and Hg in rice plant when co-present.