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Response to platelet-activating factor in human platelets stored and aged in plasma. Decrease in aggregation,

S D Shukla1, W J Morrison, D M Klachko

  • 1Department of Pharmacology, School of Medicine, University of Missouri-Columbia.

Transfusion
|July 1, 1989
PubMed
Summary
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Human platelet concentrates stored for 8 days showed maintained platelet counts but decreased platelet-activating factor (PAF) responses. Storage impacts PAF receptor function, signaling, and aggregation, indicating platelet quality decline.

Area of Science:

  • Hematology
  • Biochemistry
  • Cell Biology

Background:

  • Human platelet concentrates are stored for transfusion.
  • Platelet function is critical for hemostasis.
  • Understanding storage effects on platelet quality is essential.

Purpose of the Study:

  • To evaluate the impact of storage on human platelet concentrates.
  • To assess changes in platelet function and receptor interactions over 8 days.

Main Methods:

  • Storage of platelet concentrates in polyolefin bags at 22-24°C.
  • Aseptic sampling of platelet-rich plasma (PRP) at intervals.
  • Analysis of platelet counts, morphology (SEM), PAF-stimulated aggregation, phosphoinositide turnover, and [3H]PAF binding.

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Main Results:

  • Platelet counts remained stable over 8 days.
  • Morphology changed from biconcave to irregular shapes.
  • PAF-induced aggregation decreased significantly, with 50% loss by Day 2 and 80% loss by Day 6.
  • Phosphoinositide turnover reduced by 50% within 2-3 days and to 25% by Day 5-6.
  • [3H]PAF binding showed subtle to noticeable changes by Day 6.

Conclusions:

  • Platelet number is maintained during 8-day storage.
  • Storage leads to a general deterioration of platelet responses to PAF.
  • Changes occur at the receptor, signaling, and aggregation levels, suggesting altered receptor affinity or number.