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DNA Sequence Recognition by DNA Primase Using High-Throughput Primase Profiling
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DNA Sequence Recognition by DNA Primase Using High-Throughput Primase Profiling

Published on: October 8, 2019

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Interaction between phage BFK20 helicase gp41 and its host Brevibacterium flavum primase DnaG

Barbora Solteszova1, Nora Halgasova1, Gabriela Bukovska1

  • 1Department of Genomics and Biotechnology, Institute of Molecular Biology, Slovak Academy of Sciences, Dubravska cesta 21, 845 51 Bratislava, Slovakia.

Virus Research
|December 3, 2014
PubMed

Insights

This study investigated protein interactions between corynephage BFK20 and Brevibacterium flavum CCM 251 replication proteins. Phage protein gp41 strongly interacted with host protein DnaG, revealing key molecular mechanisms.

Area of Science:

  • Microbiology
  • Molecular Biology
  • Virology

Background:

  • Bacterial viruses, or phages, interact with host replication machinery.
  • Understanding these interactions is crucial for deciphering viral replication strategies.

Purpose of the Study:

  • To identify and characterize protein-protein interactions between corynephage BFK20 replication proteins and host Brevibacterium flavum CCM 251 replication proteins.
  • To investigate the oligomeric state of specific phage proteins.

Main Methods:

  • Bacterial two-hybrid system was employed to screen for protein interactions.
  • β-galactosidase activity assays quantified interaction strengths.
  • 2D blue native SDS-PAGE and Western blot analysis were used for further characterization.

Main Results:

  • Phage proteins gp41, gp42, and gp43 were found to self-associate, indicating they form in vivo oligomers.
  • Phage protein gp41 interacted with several host replication proteins, including DnaZX, DnaN, Dnaδ, DnaG, and SSB.
  • The most significant interaction identified was between phage gp41 and host DnaG.

Conclusions:

  • Corynephage BFK20 replication proteins engage with the host replication machinery of Brevibacterium flavum.
  • Phage protein gp41 plays a significant role in interacting with host proteins, particularly DnaG, potentially influencing viral DNA replication.
Keywords:
Bacterial two-hybrid systemBacteriophageBlue native electrophoresisCorynebacteriumProtein interactionReplication protein

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