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Hydrogen Production and Utilization in a Membrane Reactor
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An innovative membrane bioreactor for methane biohydroxylation.

N Pen1, L Soussan1, M-P Belleville1

  • 1IEM (Institut Europeen des Membranes), UMR 5635 (CNRS-ENSCM-UM2), Universite Montpellier II, Place E. Bataillon, F-34095 Montpellier, France.

Bioresource Technology
|December 3, 2014
PubMed
Summary

A novel membrane bioreactor (MBR) enhances microbial methane hydroxylation using Methylosinus trichosporium OB3b. This safe and efficient process significantly boosts productivity compared to previous methods.

Keywords:
Membrane bioreactorMethaneMethanolMethylosinus trichosporium OB3bMicrobial hydroxylation

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Area of Science:

  • Biotechnology
  • Chemical Engineering
  • Environmental Microbiology

Background:

  • Microbial methane hydroxylation is crucial for converting methane into valuable products.
  • Traditional methods face challenges with mass transfer limitations and safety concerns regarding explosive gas mixtures.
  • Methylosinus trichosporium OB3b is a well-studied methanotroph capable of methane oxidation.

Purpose of the Study:

  • To develop and demonstrate an efficient and safe membrane bioreactor (MBR) for microbial methane hydroxylation.
  • To improve the mass transfer of gaseous substrates (methane and oxygen) in the bioreaction.
  • To achieve high productivity in methane hydroxylation using Methylosinus trichosporium OB3b.

Main Methods:

  • Development of a novel MBR system coupling a bioreactor with two gas/liquid macroporous membrane contactors.
  • Operation of the MBR in fed-batch mode for controlled substrate supply.
  • Cultivation of Methylosinus trichosporium OB3b for methane hydroxylation.

Main Results:

  • Demonstrated feasibility and reproducibility of the novel MBR process.
  • Achieved a mass transfer rate twice that of a conventional batch reactor.
  • Reached a high productivity of 75±25 mg methanol/(g dry cell·h), significantly outperforming previous MBRs.
  • Avoided the risk of explosive gas mixture formation inherent in bubble-sparger systems.

Conclusions:

  • The developed MBR offers an efficient and safe platform for microbial methane hydroxylation.
  • Macroporous membrane contactors enhance gas-liquid mass transfer, leading to improved productivity.
  • This MBR technology presents a promising alternative to conventional bioreactors for methane conversion applications.